Hi to all group members

Srikanth Aluru @Srikanth_Aluru4

10 October 2010 0 10K Report

Here I would like to ask you a question Regarding continuation of desired work on Diagnostic tool development

Project title: Avian flu Neuraminidase gene expression ,purification and quantification

from here onwards I am trying to elongate the work on Bio physical levels like Structural developments

So please post your comments on further process develpment of project

Badges
Science topic

More Srikanth Aluru's questions See All

Immunofluroscence LC3 protocols and conditions?

Hi I am looking for an optimized protocol for Lc3 and lamp 2 immunofluroscene.currently facing a problem,,have been tried with different conditions as per few papers,but could not able to make...

06 July 2014 8,142 4 View

No title

Hello, Does anyone sucessed to make THP1 cell transfection efficiency? I am currently trying to tranfect THP1 adherent cells (PMA differentiated for 10 hrs) with specific vendor protocol. But it...

06 July 2014 2,515 8 View

What is the difference between co-infections and mixed infections?

Do we consider a co-infection as invitro infection of two different pathogens to a single cell type, or infection of two different pathogens to an animal model. Like wise Mixed infection-the term...

08 September 2013 6,289 10 View

Iron homeostasis and Intracellular microbial defense

As a bonafide statement, Iron is an important sustain for the colonization of microbes in the host. Targeting microbial iron acquisition system may also a crucial therapeutic way to fight against...

06 July 2013 2,686 0 View

How does an inhibitory signal cascade re-activate (how the downregulation cascade reactivates for upregulation)?

How does an inhibition complex reactivate again for its own signal transduction. I am interested to know the possibilities of a kinetic mechanism of signaling cascade of inhibitory complex. We...

04 May 2013 7,675 0 View

What is the follow up method in alignment of amino acid sequence?

I would like to know how you align the amino acid sequences from different databases?For example, if I wish to know about cytoplasmic domains of TLR's. I am not aware of how to generate the...

04 May 2013 1,624 2 View

What is the standard method for assay co-infection experiments in vitro?

I am trying to perform a co-infection experiment on activated macrophage and I want to know discrimination of Immune regulations. What kind of experimental assaying outlines can resolve my...

01 February 2013 4,919 2 View

Research has deviations in its mode? Why we RE-search?

As a mind of philosopher-research has deviations in its mode with an initial level of thinking,formulating and hypothesis.But all hypothesis are not a well defined laws. All hypothesis will...

11 December 2011 6,808 0 View

Intrested on Research ,Specially expression studies of Recombinant proteins in Prokaryotic Sysems And Quantification of desired proteins by Biophysical techniques

Hi . Here Srikanth, A student of Life science having lots of Practical Ideas.. Currently looking to take a chance

09 October 2010 6,567 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View