Hi .
Here Srikanth, A student of Life science having lots of Practical Ideas..
Currently looking to take a chance
Hi I am looking for an optimized protocol for Lc3 and lamp 2 immunofluroscene.currently facing a problem,,have been tried with different conditions as per few papers,but could not able to make...
06 July 2014 8,251 4 View
Hello, Does anyone sucessed to make THP1 cell transfection efficiency? I am currently trying to tranfect THP1 adherent cells (PMA differentiated for 10 hrs) with specific vendor protocol. But it...
06 July 2014 2,660 8 View
Do we consider a co-infection as invitro infection of two different pathogens to a single cell type, or infection of two different pathogens to an animal model. Like wise Mixed infection-the term...
08 September 2013 6,420 10 View
As a bonafide statement, Iron is an important sustain for the colonization of microbes in the host. Targeting microbial iron acquisition system may also a crucial therapeutic way to fight against...
06 July 2013 2,784 0 View
How does an inhibition complex reactivate again for its own signal transduction. I am interested to know the possibilities of a kinetic mechanism of signaling cascade of inhibitory complex. We...
04 May 2013 7,770 0 View
I would like to know how you align the amino acid sequences from different databases?For example, if I wish to know about cytoplasmic domains of TLR's. I am not aware of how to generate the...
04 May 2013 1,726 2 View
I am trying to perform a co-infection experiment on activated macrophage and I want to know discrimination of Immune regulations. What kind of experimental assaying outlines can resolve my...
01 February 2013 5,001 2 View
As a mind of philosopher-research has deviations in its mode with an initial level of thinking,formulating and hypothesis.But all hypothesis are not a well defined laws. All hypothesis will...
11 December 2011 6,899 0 View
Here I would like to ask you a question Regarding continuation of desired work on Diagnostic tool development Project title: Avian flu Neuraminidase gene expression ,purification and...
09 October 2010 9,986 0 View
Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore
04 June 2024 5,688 2 View
The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...
12 March 2024 3,926 0 View
Hi, We are trying to isolate rat MSCs from Fischer F455 rats. So far we tried a femur flush protocol to flush the bone marrow into a petri dish. However, we are wondering if we should do...
12 July 2023 4,684 0 View
Call of Edotrial boards in Algerian Journal of Biosciences About more informations please visit the journal website https://www.journal.acse.science/index.php/ajb/index If you are interested...
17 April 2023 3,999 0 View
In the field of biosciences we often get in contact with the questions: Are viruses alive, a form of life? Is fire a form of life? Stars also burn fuel and stay stable until their fuel is burned...
16 April 2023 7,233 1 View
I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...
12 January 2023 4,248 2 View
The protein was extracted from mouse tissue(brain),marker is Thermo 26619. The ladder was about in 25kd.And the antibody is BD Bioscience#asyn antibody. I'm not sure if it was α-synuclein.
08 December 2022 9,772 4 View
I've grown my influenza straind in MDCK cells and have isolated them. Now I have to sequence my virus, my lab uses Eton Biosciences. Do I send in primers for each gene? Do I extract RNA from the...
18 May 2022 2,592 0 View
The reporter cell line has been cultured following the suppliers recommendation (see below): Thaw Medium 1 (BPS Bioscience #60187): MEM medium (Thermo Fisher, #11095098) supplemented with 10% FBS...
09 January 2022 3,186 0 View
Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...
05 October 2020 1,928 2 View