10 October 2010 0 7K Report

Hi .

Here Srikanth, A student of Life science having lots of Practical Ideas..

Currently looking to take a chance

More Srikanth Aluru's questions See All
Immunofluroscence LC3 protocols and conditions?

Hi I am looking for an optimized protocol for Lc3 and lamp 2 immunofluroscene.currently facing a problem,,have been tried with different conditions as per few papers,but could not able to make...

06 July 2014 8,251 4 View

What are the THP1 Adhérent cells/ Transfection Protocols?

Hello, Does anyone sucessed to make THP1 cell transfection efficiency? I am currently trying to tranfect THP1 adherent cells (PMA differentiated for 10 hrs) with specific vendor protocol. But it...

06 July 2014 2,660 8 View

What is the difference between co-infections and mixed infections?

Do we consider a co-infection as invitro infection of two different pathogens to a single cell type, or infection of two different pathogens to an animal model. Like wise Mixed infection-the term...

08 September 2013 6,420 10 View

Iron homeostasis and Intracellular microbial defense

As a bonafide statement, Iron is an important sustain for the colonization of microbes in the host. Targeting microbial iron acquisition system may also a crucial therapeutic way to fight against...

06 July 2013 2,784 0 View

How does an inhibitory signal cascade re-activate (how the downregulation cascade reactivates for upregulation)?

How does an inhibition complex reactivate again for its own signal transduction. I am interested to know the possibilities of a kinetic mechanism of signaling cascade of inhibitory complex. We...

04 May 2013 7,770 0 View

What is the follow up method in alignment of amino acid sequence?

I would like to know how you align the amino acid sequences from different databases?For example, if I wish to know about cytoplasmic domains of TLR's. I am not aware of how to generate the...

04 May 2013 1,726 2 View

What is the standard method for assay co-infection experiments in vitro?

I am trying to perform a co-infection experiment on activated macrophage and I want to know discrimination of Immune regulations. What kind of experimental assaying outlines can resolve my...

01 February 2013 5,001 2 View

Research has deviations in its mode? Why we RE-search?

As a mind of philosopher-research has deviations in its mode with an initial level of thinking,formulating and hypothesis.But all hypothesis are not a well defined laws. All hypothesis will...

11 December 2011 6,899 0 View

Hi to all group members

Here I would like to ask you a question Regarding continuation of desired work on Diagnostic tool development Project title: Avian flu Neuraminidase gene expression ,purification and...

09 October 2010 9,986 0 View

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The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...

12 March 2024 3,926 0 View

Isolation of rat mesenchymal stem cells (Fischer F455 rats) - protocol and surface markers?

Hi, We are trying to isolate rat MSCs from Fischer F455 rats. So far we tried a femur flush protocol to flush the bone marrow into a petri dish. However, we are wondering if we should do...

12 July 2023 4,684 0 View

Call of Editorial boards at Algerian Journal of Biosciences?

Call of Edotrial boards in Algerian Journal of Biosciences About more informations please visit the journal website https://www.journal.acse.science/index.php/ajb/index If you are interested...

17 April 2023 3,999 0 View

Philosophically, are stars alive?

In the field of biosciences we often get in contact with the questions: Are viruses alive, a form of life? Is fire a form of life? Stars also burn fuel and stay stable until their fuel is burned...

16 April 2023 7,233 1 View

Where to purchase viruses other than ATCC?

I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...

12 January 2023 4,248 2 View

Is it true that the molecular weight of asyn protein is 25kd in westen-blot ?

The protein was extracted from mouse tissue(brain)，marker is Thermo 26619. The ladder was about in 25kd.And the antibody is BD Bioscience#asyn antibody. I'm not sure if it was α-synuclein.

08 December 2022 9,772 4 View

How do I sequence my isolated virus?

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18 May 2022 2,592 0 View

My TCF/LEF Reporter - HEK293 Cell Line (Wnt Signaling Pathway) is struggling to grow upon thawing. Has anyone had similar issue with this cell line?

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Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences?

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...

05 October 2020 1,928 2 View

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