Immunofluroscence LC3 protocols and conditions?

Hi I am looking for an optimized protocol for Lc3 and lamp 2 immunofluroscene.currently facing a problem,,have been tried with different conditions as per few papers,but could not able to make...

06 July 2014 8,142 4 View

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Hello, Does anyone sucessed to make THP1 cell transfection efficiency? I am currently trying to tranfect THP1 adherent cells (PMA differentiated for 10 hrs) with specific vendor protocol. But it...

06 July 2014 2,515 8 View

What is the difference between co-infections and mixed infections?

Do we consider a co-infection as invitro infection of two different pathogens to a single cell type, or infection of two different pathogens to an animal model. Like wise Mixed infection-the term...

08 September 2013 6,289 10 View

Iron homeostasis and Intracellular microbial defense

As a bonafide statement, Iron is an important sustain for the colonization of microbes in the host. Targeting microbial iron acquisition system may also a crucial therapeutic way to fight against...

06 July 2013 2,686 0 View

How does an inhibitory signal cascade re-activate (how the downregulation cascade reactivates for upregulation)?

How does an inhibition complex reactivate again for its own signal transduction. I am interested to know the possibilities of a kinetic mechanism of signaling cascade of inhibitory complex. We...

04 May 2013 7,675 0 View

What is the follow up method in alignment of amino acid sequence?

I would like to know how you align the amino acid sequences from different databases?For example, if I wish to know about cytoplasmic domains of TLR's. I am not aware of how to generate the...

04 May 2013 1,624 2 View

What is the standard method for assay co-infection experiments in vitro?

I am trying to perform a co-infection experiment on activated macrophage and I want to know discrimination of Immune regulations. What kind of experimental assaying outlines can resolve my...

01 February 2013 4,919 2 View

Research has deviations in its mode? Why we RE-search?

As a mind of philosopher-research has deviations in its mode with an initial level of thinking,formulating and hypothesis.But all hypothesis are not a well defined laws. All hypothesis will...

11 December 2011 6,808 0 View

Hi to all group members

Here I would like to ask you a question Regarding continuation of desired work on Diagnostic tool development Project title: Avian flu Neuraminidase gene expression ,purification and...

09 October 2010 9,882 0 View

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences?

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...

05 October 2020 1,713 2 View

Do you agree with the just issued Great Barrington Declaration regarding the response to COVID-19?

The short and to-the-point text is here and I for one gladly co-signed this declaration, just as I also co-signed the balanced approach statement issued in July 2020 by 23 public health experts...

04 October 2020 8,795 11 View

The meaning of protein phosphorylation to bioscience?

Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote. So did...

23 July 2020 7,135 3 View

Is there any cause of pellet from the culturing media?

11 June 2020 3,585 3 View

How do you reduce the static forces in canon ball packing of fruits for transportation?

Examples of how to increase the contact surface area of the bottom area? Thank you.

25 March 2020 9,187 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

03 January 2020 3,565 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

31 December 2019 3,792 4 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

22 September 2019 2,889 3 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

08 September 2019 5,009 3 View

So what you think that either you will choose to develop a desktop application, mobile application or the application that can run on both platforms ?

As internet usage increase web application development become a challenge, because it is mostly customer requirement that web application must be usable on both desktop computer and...

07 August 2019 1,193 3 View