Srikanth Aluru

10 Questions 45 Answers 0 Followers

Questions related from Srikanth Aluru

Immunofluroscence LC3 protocols and conditions?

Hi I am looking for an optimized protocol for Lc3 and lamp 2 immunofluroscene.currently facing a problem,,have been tried with different conditions as per few papers,but could not able to make...

07 July 2014 8,194 4 View

What are the THP1 Adhérent cells/ Transfection Protocols?

Hello, Does anyone sucessed to make THP1 cell transfection efficiency? I am currently trying to tranfect THP1 adherent cells (PMA differentiated for 10 hrs) with specific vendor protocol. But it...

07 July 2014 2,614 8 View

What is the difference between co-infections and mixed infections?

Do we consider a co-infection as invitro infection of two different pathogens to a single cell type, or infection of two different pathogens to an animal model. Like wise Mixed infection-the term...

09 September 2013 6,361 10 View

Iron homeostasis and Intracellular microbial defense

As a bonafide statement, Iron is an important sustain for the colonization of microbes in the host. Targeting microbial iron acquisition system may also a crucial therapeutic way to fight against...

07 July 2013 2,738 0 View

How does an inhibitory signal cascade re-activate (how the downregulation cascade reactivates for upregulation)?

How does an inhibition complex reactivate again for its own signal transduction. I am interested to know the possibilities of a kinetic mechanism of signaling cascade of inhibitory complex. We...

05 May 2013 7,731 0 View

What is the follow up method in alignment of amino acid sequence?

I would like to know how you align the amino acid sequences from different databases?For example, if I wish to know about cytoplasmic domains of TLR's. I am not aware of how to generate the...

05 May 2013 1,676 2 View

What is the standard method for assay co-infection experiments in vitro?

I am trying to perform a co-infection experiment on activated macrophage and I want to know discrimination of Immune regulations. What kind of experimental assaying outlines can resolve my...

02 February 2013 4,966 2 View

Research has deviations in its mode? Why we RE-search?

As a mind of philosopher-research has deviations in its mode with an initial level of thinking,formulating and hypothesis.But all hypothesis are not a well defined laws. All hypothesis will...

12 December 2011 6,862 0 View

Hi to all group members

Here I would like to ask you a question Regarding continuation of desired work on Diagnostic tool development Project title: Avian flu Neuraminidase gene expression ,purification and...

10 October 2010 9,938 0 View

Intrested on Research ,Specially expression studies of Recombinant proteins in Prokaryotic Sysems And Quantification of desired proteins by Biophysical techniques

Hi . Here Srikanth, A student of Life science having lots of Practical Ideas.. Currently looking to take a chance

10 October 2010 6,637 0 View