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Good morning. I have recently switched computers and gotten a new edition of Graphpad. I am trying to open a very large file containing all of my graphs for a project, but am encountering an...
22 March 2020 6,810 1 View
I have an established cell line that expresses GFP constitutively. Once I transfect the cell line, there shouldn't any GFP expression since the DNA payload doesn't have a fluorescence marker. My...
23 September 2019 3,940 1 View
Good evening - I need to come up with a project idea for co-op. The project needs to be mostly FACS-related but also give exposure to other techniques, such as cell culture and molecular biology...
18 September 2019 8,911 2 View
Hi everyone! I was wondering if anyone might have any ideas of how to conduct some potential analyses for some research I am working on. I am interested in the moderating role of trait emotion...
09 May 2019 5,118 3 View
About connecting SAP with matlab
16 November 2018 2,663 3 View
I want to link matlab to opensees to complete the dynamic stability analysis of a 2D frame
13 August 2018 3,721 4 View
Dear all, Anybody could explain to me which method is better for measurement the level of toll like receptor protein in tumor tissue , Immunohistochemistry vs western blot? Thanks in advance.
09 April 2018 6,343 5 View
Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...
15 March 2018 8,141 3 View
My lab is interested in extracting RNA from sperm (small RNAs specifically), and I believe we have the correct kit (Zymo Quick-RNA Micro-prep), but we already have a lot of trouble with extracting...
06 August 2017 8,639 1 View
05 August 2017 5,723 5 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
I want to deposit multilayer coating of germanium and silicon monoxide on silicon wafer using ebeam evaporation?
21 February 2021 2,141 3 View
I've landed on needing some type of highly optically transparent film or cover material that prevents evaporation of culture media; I am not sure which subfield of materials science would be able...
08 February 2021 5,760 1 View
I have to simulate a hydrometallurgical process where there are the following phases: - leaching (solid-liquid reactions) - cementation (solid-liquid reactions) - electrowinning (energy ) -...
30 January 2021 9,225 3 View
20 January 2021 6,322 2 View
I purified my Boc-protected molecule using HPLC. The eluent is ACN/H2O/(0.05% TFA) . After I evaporated the solvent (65 oC), the product was collect as the MS result showed ~20% of the product was...
14 January 2021 5,487 5 View
By that I mean, for example, I was a researcher in solar cell materials, and currently, I want to research the study of evaporation in soil and water, i.e. in the processes of solving water...
10 January 2021 6,499 4 View
Hello, Setting: We have a rather large dataset consisting of somewhat 500 samples containing metabolomics data. The data was obtained by analysing dry capillary blood using HPLC methods, yielding...
04 January 2021 8,773 5 View
I want to remove solvent to obtain a solid . i would like to remove solvents such as THF, TEA, carbon tetrachloride . the obtained solid which is a Phosphorus ester. What could be an suitable...
04 January 2021 1,220 1 View