Comercial "stratum corneum substitute" lipid mixture?

Greetings We want to look at the effect of Stratum Corneum composition on bacterial attachment. Does anyone know a commercially available "stratum corneum substitute" lipid mixture? Or is willing...

17 November 2019 1,383 2 View

Extraction of Heme A, Heme B and Heme O?

Greetings I am looking for a trusted protocol for extraction of Heme A, Heme B and Heme O, to be used as standards in HPLC analysis. Alternatively if you know of a commercial source also...

27 May 2019 5,240 2 View

Can I connect HP1047A RI detector to Agilent 1200 HPLC system?

Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....

19 October 2017 6,270 9 View

Q-Dots or other high yield fluorophore that exite at ~254nm and emit at ~300nm?

Greetings. I am looking for Q-Dots or other high yield fluorophore that exite at ~254nm and emit at ~300nm. Any ideas welcome Yoram

27 March 2016 9,342 2 View

Can anyone answer my question in hydrochemistry of boreal wetlands?

I am working on time series in chemical composition of shallow ground water samples collected between 1994 and 2010 at the largest known boreal wetland. Aside from the understandable and well...

15 June 2014 2,792 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

What do you think about the stability of redox active ketoesters? Can I isolate this compounds?

Although I want to synthesize a redox active ketoester like 1,3-dioxoisoindolin-2-yl 2-oxo-2-phenylacetate, I have no idea about the stability of this kind of compounds. I know that some redox...

19 January 2021 5,672 3 View

Can anyone suggest an RNA extraction protocol from grape berries?

I am trying to extract RNA, from grape berries of a red variety, for qPCR (to study gene expression). I have tried nucleozol and a CTAB protocol (with b-mercaptoethanol+boric acid + cleaning with...

18 January 2021 8,857 2 View

Work up of ester?

What is the detailed procedure for work up in ester synthesis from acid chloride and alcohol? What organic solvent to use for extraction?

18 January 2021 8,649 6 View

No title

16 January 2021 8,334 5 View

Would you tell me how to dissolve cholesterol in RPMI medium?

We are planning to add cholesterol to cells in order to raise the intracellular cholesterol concentration and confirm the protein expression of SREBP2. I didn't know if I should dissolve...

14 January 2021 871 6 View