Dear research community,
I'm beginning to work with the renal rat cell line PC12 and the information I collected so far are confusing.
I read about the doubling time of the cells reaching from 48h to 90h, which is quite a difference when planning experiments.
Also, I got two medium suggestions based on RPMI-1640 and the other on DMEM.
What are your experiences with cell doubling time and media? Does the medium have an influence on the doubling time?
For starters, I just want to cultivate the PC12 cells in suspension and later on differentiate them with NGF.
Looking forward to your answers :)
Dear Julia Claussen ,
I used RPMI 1640 liquid medium supplemented with 10% Donor Horse serum, 5% Fetal Bovine Serum, 1% penicillin/streptomycin, and 1% L-glutamine. Doubling time was about 90 h in suspension. If you want to speed up doubling time, I recommended that you should use the flask in an upright position, not in a horizontal position.
good luck
I also recorded DT values ranging from 40 to 92 hours in the relevant Cellosaurus entry:
https://www.cellosaurus.org/CVCL_0481
But while looking if I could update the entry with more data points on the doubling time and especially the influence of culture medium, I stumbled on this very recent publication:
https://pubmed.ncbi.nlm.nih.gov/34348336/
And its a bit worrisome as they compare PC12 cells from 5 different sources (4 labs and ATCC) and they conclude "The results show that the 5 cell lines are very variable in terms of shape, proliferation rate, motility, adhesion to the substrate and gene expression."
Best regards
Amos Bairoch
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