Simge Unay

6 Questions 19 Answers 0 Followers

Questions related from Simge Unay

What could be causing the PC12 (CRL-1721) cell line to exhibit adherent characteristics?

I have been working with the PC12 (CRL-1721) cell line since 2020. Although these cells are typically suspension cells, they have recently started to exhibit adherent characteristics, and I am no...

07 July 2025 9,233 0 View

How to specifically stain the mitochondria dysfunction by IHC in neuronal cells?

Dear researchers, Could we use immunohistochemistry method to somehow specifically stain the pathologic change of mitochondria on NGF-PC12 or DRG cells? Is there any specific markers for...

29 January 2023 3,137 2 View

Has anyone used the LDH assay kit (MAK066-sigma)?

Hi, In order to measure the LDH level in the experimental groups in Pc12 cells, I tried the lactate dehydrogenase activity assay kit (MAK066-sigma). I used one on the brand's page for the...

21 November 2021 10,062 3 View

Why do PC12 cells die after 100ng/ml NGF for 72 h incubation?

Hi, I applied on PC12 cells 100 ng/ml NGF and incubated for 72 hours to differentiation. After 72 h, approximately most cells differentiate and then I applied an anticancer drug but all of the...

14 September 2021 4,214 6 View

Polylysine-coated tissue culture surfaces?

Hello everyone. I would like to coat coverslips/plates with poly-D-lysine. But there are so much different protocols about incubation times. Do you know standard protocol?

24 May 2020 9,896 0 View

How to choose control in MTT assay calculation?

Dear researchers, I have question about MTT control? I applied ultrasound to cells with photosensitive agent and microbubbles. To MTT test, which do i should take for control? Will it cells...

10 October 2019 6,936 1 View