Do u have any tips for blotting of integrin b1?

I blocked with skimmed milk, did the 1ry Abs incubation overnight at 4C. I have used two Abs (two membrane) for detection of integrin b1 using western blotting. Washed 5 times with TBST, each is...

30 November 2020 4,236 3 View

How can I extract high quality bacteria genomic DNA from sulfate reducing bacteria (SRB)?

All step from culture bacteria, which is the suitable media, and the age of bacteria.

22 September 2019 244 1 View

In NAMD, should the flexible cell option turned on during NPT simulation ?

10 May 2019 6,220 4 View

Can anyone assist me with Incremental Conductance MPPT Algorithm Simulink Model?

I wanna Simulink Model in detailed for Incremental Conductance MPPT Algorithm for Single Phase Standalone PV system.

22 March 2019 7,280 3 View

How to prepare E. coli for SEM image?

I'm developing some protein surface displaying system that works in E. coli, I need to take SEM images shows successful displaying of some peptides on the surface of E. coli. However I don't...

20 March 2019 8,209 1 View

Hi..,can anyone help me to count blastomeres in an embryo image on day 3 (that's with 8 cell) ??????

here is attached images that i want to count cells on >>>if there is a Matlab code do this????

15 March 2018 3,055 6 View

What is the meaning that cancerstemcell ratio do not change by treatment with herbal extract?

what is the meaning that cancerstemcell ratio do not change by treatment with herbal EXTRACT?

01 December 2017 7,994 2 View

Why number of cancerstemcells increase from .5% to16.5% in the presence of herbal extract (that expected to decrease cancerstemcells)

Why number of cancerstemcells increase from .5% to16.5% in the presence of herbal extract ( that expected to decrease number of cancerstemcells)

02 August 2017 1,857 3 View

If i used polymer or composite as coating for metal or alloy

if i used polymer or composite as coating for metal or alloy when i calculate the corrosion current by tafel extrapolation we need density and equivalent weight for calculation in this case...

15 May 2017 6,454 3 View

How can I kill E. coli and keep its protein?

I'm trying to synthesize protein outside the bacterial cell wall. I would check its function in a dead E. coli bodies. So I'm trying to find the best way to kill E. coli without affecting this...

05 April 2017 3,655 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View