Is it possible for the same compound but origins from two different pathways (one synthetic and the other is biological) behave slightly different on LC in terms of retention time?
Hi Emad
On the level of GC analysis I have the experience to say that:
The retention times of both synthetic and natural compounds are the same
I think that in case of LC it should be also the same, but i hope that a colleague who have some expertise in LC can answer that question too
Good luck
Amr
Dear Emad, the separation of a compound in LC or GC chromatography is strictly related to the structure of the compound and its interaction with the mobile and stationary phases. Define "slightly different", variations in Rt may derive from inconsistent chromatographic conditions. If I were you, I would run 3-4 times the same compound under the same conditions to check the stability of my HPLC.
Spyros is right: This should be excluded by different analyses and calculation of mean and standard deviation. If there is really a difference in retention times, then there is a difference in the compounds. For example, if you are analysing proteins, different retention times could have the reason of different tertiary structure, if no modification is the source.
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