What is the difference between genbank , bankit and clinvar database?

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The importance of humanistic care?

Humanistic care is mainly about caring for people's living conditions, affirming people's living conditions and dignity, and pursuing human freedom and liberation. With the continuous development...

10 July 2024 5,107 2 View

My EMSA Blot is not sharp and blurry after exposure with a Phosphorimager (Typhoon). What can I do to fix that?

I'm currently trying to do some EMSA using P32. The first two gels looked perfect, with sharp bands and no background. However, all the following ones looked like the blot below. I'm not doing...

18 June 2024 1,967 2 View

Can sample size differ across paths in a path analysis?

Hello there! I'm looking at a mediation model with 2 predictors, 2 mediators and 3 outcomes. My full sample size is 558, but one of those outcomes only has data for 410 participants. So when I...

18 May 2024 1,404 4 View

Authenticity of "Plasma Science and Technology" Journal?

Dear colleagues, I require urgent assistance regarding the authenticity of the "Plasma Science and Technology" journal. Upon investigation, I found two websites bearing the same name and ISSN...

17 April 2024 5,732 2 View

How can I prepare a sample of green coffee extract obtained by supercritics to be analyzed by HPLC?

I would like to know how I can prepare my sample of supercritical extract of green coffee beans if I want to analyze chlorogenic acid and I need to prepare the sample to analyze it by HPLC. I...

31 March 2024 854 2 View

I tried to precipitate my Protein with using iced acetone left it in ice but there is no pellet ?

I used the iced acetone in -20C , used 4 times double the volume of my protein, and incubated the mixture inside the ice for more than 3 hours and I didn't notice any pellet.

22 February 2024 7,424 0 View

What is this structure in colorectal cancer cell line?

Hi, I could see this morphology under the microscope (black arrow, 20x). Could someone identify what this morphology/ structure could be? and if so how to stain it with a specific dye? Cells are...

21 February 2024 9,450 4 View

How can I seed 0.5x10^6 cells per well in a 96-well plate?

How can I seed 0.5x10^6 cells per well in a 96-well plate (I only need to seed 5 wells)? I have a T25 flask (5 mL total volume) with a cell density of 0.6x10^6 cells/mL.

17 February 2024 5,535 1 View

What should I do if my polyacrylamide gels have this stain?

In this electrophoresis , i'm using serum samples without any treatment, mixing them with sample buffer in a 1:1 ratio. Te run is performed at 150v for 1 Hour, an this is the result after...

14 November 2023 2,732 5 View

What is the best research topic for integration of AI ( DL) with lean six sigma in order to improve operational control?

I will be using it for DBA thesis

04 August 2023 8,237 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Any suggestions for and either free or cheap DNA sequence editing program?

A need to analyse specific sequences gathered from genbank in order to analyse primers matching/designing. I have used Sequencher (gene codes) in the past and I loved it. Yet prices are now...

24 April 2024 259 3 View

How much time does NCBI GenBank takes for public release of complete chloroplast genome sequence?

We have assembled a chloroplast genome of angiosperm and submitted the annotated assembly to NCBI GenBank. Within two-working days we got accession number for that submission. GenBank in their...

21 April 2024 9,974 0 View

Can anyone share insights or provide the complete mRNA sequence for tdtomato-WPRE for scRNA seq analysis?

Hello all, I'm currently immersed in research involving the Rosa26-tdtomato (Ai9) mouse line. We performed scRNA seq and analysis focusing on tdtomato-expressing cells but faced a challenge with...

01 February 2024 6,390 0 View

How to download nucleotide sequences as a gbk file?

Hello, I need to use a program that only takes gbk as input. I am working with viral sequences that are not under "genomes" but under "nucleotide" category in genbank. I need to compare those with...

24 October 2023 5,620 1 View

How can I compare the protein sequence from multiple genomes deposited in GenBank?

I would like to compare the sequence of my protein of interest within all of the Staphylococcus saprophyticus genomes that are deposited in GenBank. How can I extract this information without...

16 October 2023 784 1 View

How to fix GenBank Submission Error: CDS has frameshift?

I got a reply from my sequence submission to GenBank saying that "high confidence possible frameshift in CDS (frame not restored before end) [cause:insert,S:503(1),M:742; frame:;...

21 September 2023 336 0 View

Can anyone pls suggest to me "how to merge multiple Genbank records (different accession numbers) in a single file to merge into a single file?

I have a file with multiple genbank records contianing different accession umbers. A single file contains different plasmid gbff records. I want all the plasmid records to be comnined into single...

20 September 2023 350 0 View

Facing a syntax error problem wile running brig with genbank file what's the solution?

I am facing a problem while running brig with genbank files. I want to give gene annotations & following the manual I have added the genes with the start & stop codon mentioned. But after...

05 August 2023 5,601 0 View

Facing a syntax error problem while running BRIG with genbank file what's the solution?

I am facing a problem while running BRIG with genbank files. I have given plasmid sequences as my input files. I want to give gene annotations & following the manual I have added the genes...

05 August 2023 809 0 View