I tried to precipitate my Protein with using iced acetone left it in ice but there is no pellet ?

23 February 2024 0 7K Report

I used the iced acetone in -20C , used 4 times double the volume of my protein, and incubated the mixture inside the ice for more than 3 hours and I didn't notice any pellet.

Badges
Science topic

Similar topics
Engineering
Modeling

More Shoruq Naji's questions See All

Authenticity of "Plasma Science and Technology" Journal?

Dear colleagues, I require urgent assistance regarding the authenticity of the "Plasma Science and Technology" journal. Upon investigation, I found two websites bearing the same name and ISSN...

17 April 2024 5,732 2 View

What is the best research topic for integration of AI ( DL) with lean six sigma in order to improve operational control?

I will be using it for DBA thesis

04 August 2023 8,236 1 View

How to choose between Pearson and Spearman correlation?

Hi, I have 100 samples and want to find the correlation between the clinical characteristics of the samples. Can someone help me how to determine which one to use? I really appreciate any help...

03 June 2023 1,788 4 View

How to find chi-square for single categorical variable?

Hi, I have a total number of examined participants 642; the infected number is 61, and the Non-infected 581; any idea how to find the chi-square and p-value? regards

30 May 2023 7,161 5 View

Does anyone have a journal citation report of 2023?

Hi I need to make sure of the journal that I want to submit a manuscript is not fake. I have the journal citation report for 2022 but I need a journal citation report for 2023. regards

03 May 2023 9,896 3 View

How to Compare your sequence to the reference sequence in NCBI?

Hi, I have sequence data and I have no idea how to compare them with reference sequences in NCBI to create a phylogenetic tree. is there any educational video or a way that someone can...

04 February 2023 6,196 6 View

What is minimum and maximum number of valid Odds Ratios?

Hi, I know that the odd ratio ( OR=1 Exposure does not affect odds of outcome, OR>1 Exposure associated with higher odds of outcome and OR

31 January 2023 8,676 3 View

How to create phylogenetic tree for accession number data?

I have received accession number data from NCBI, anyone can help how to create a Phylogenetic tree, I got all the data from the same organism and the same gene, I was thinking to make the tree...

18 January 2023 2,287 3 View

What is the difference between genbank , bankit and clinvar database?

Hi, I need to know if there is a difference between Genbank, bankit and clinvar database in submitting sequence data. regards Lina

02 January 2023 6,076 3 View

How to submit PCR sequence to get an accession number in ncbi?

Hi there, I have a PCR sequence for the SNP of a particular gene, and I want to submit it to NCBI, so I need to know how to submit the sequence to NCBI to get an accession number online. Is there...

07 December 2022 1,580 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,492 1 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How to determine tannin content in fodder pellets?

22 July 2024 8,162 3 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

15 July 2024 9,025 3 View

65% saturation of ammonium sulfate in 100 ml? (for protein precipitation)?

Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...

09 July 2024 5,508 0 View

How to disperse silverparticles in water?

I synthesize silver nanoparticles ，and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...

09 July 2024 7,335 0 View