Hi,
I could see this morphology under the microscope (black arrow, 20x). Could someone identify what this morphology/ structure could be? and if so how to stain it with a specific dye?
Cells are colorectal cancer cell line, this structure does not stain with DAPI.
Thanks!
For me this looks like prominent nucleoli, which usually don't stain with DAPI.
Thanks a lot for your reply. May I ask, how can we confirm it? and will appreciate if you can recommend an article or so to know what might be the causes of the prominent nucleoli.
A simple google search will lead you to several commercial kits for staining nucleoli. I am not familiar with the types of nucleoli in colorectal cancer, but I think that's typical; as you are a PostDoc I am sure you can figure that out yourself or ask a specialist in the field.
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