Hello all,
I'm currently immersed in research involving the Rosa26-tdtomato (Ai9) mouse line. We performed scRNA seq and analysis focusing on tdtomato-expressing cells but faced a challenge with a lower-than-expected number of positive cells.
Our alignment process, using the tdtomato sequence (Genbank accession: AY678268), seems insufficient. Fellow researchers suggest success in similar cases by incorporating the full mRNA sequence, including the 3´UTR, along with tdtomato-WPRE mRNA.
I searched everywhere for the full mRNA sequence but couldn't find it. Can anyone share insights or provide the complete mRNA sequence for tdtomato-WPRE? This would greatly enhance our alignment process and improve the accuracy of tdtomato positive cell detection.
Appreciate your contributions to advancing our research!
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