How to compute the phase from a fringe pattern using Hilbert Huang Transform in MATLAB?

I have computed line Wise IMF from the fringe data using empirical mode decomposition (EMD). For each line Segment I have more than five IMF sets but i am not able to understand that how to...

10 January 2021 5,036 1 View

Are there empirical correlations available that relate data from grain size distribution curve to the shear strength of soil?

Similar to the Hazen formula for estimating permeability from the gradation curve of soil, can we identify the angle of shearing resistance of silty sand and sandy silt type of soil?

03 December 2020 6,747 3 View

Alternative method of Permeablization in IL17A assay by flowcytometry?

I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the...

01 December 2020 4,553 1 View

Suggestions how to get Shapefile of India with all 700+ Indian districts?

I need shapefile for all 700+ districts of India. From where I can find the India shapefile with all districts. Also, suggest how to get the latest India shapefile with all state and union...

07 August 2020 3,449 5 View

PSO, ABC or GA, which one to choose while solving Mixed Integer Programming problem?

I am interested to solve a mathematical problem (MILP) using evolutionary algorithms but confuse about which one to choose as a beginner in the programming languages. Suggest an algorithm easy to...

13 July 2020 4,027 51 View

What is the best tool for adding references (in a research paper) within a word file?

What is the best tool for adding references (in a research paper) within a word file

16 June 2020 8,275 9 View

How to apply nodal displacements in radial direction in ABAQUS?

I am simulating wave propagation in a plate. In that process, I need to apply nodal displacement in the radial direction at the actuator position. I entered the wave excitation signal in the...

11 June 2020 9,109 3 View

About my manuscript status?

My manuscript status shows "With Editor" after initial submission. Then, (after two weeks) it takes "Editor invited". After one and half months it backs again to the first step i.e., "With Editor"...

03 March 2020 9,065 10 View

How can I develop an ANFIS model for WQI in matlab?

I want to implement the Water quality index in ANFIS. I have used constant output, but not getting the desired output.

06 February 2020 3,394 4 View

Can food supply chains be secured by blockchain diffusion???

Blockchain technology promises to provide complete information security and elimination of intermediary alteration. But can we really full utilize this feature to food supply chain. How can we...

03 May 2019 5,476 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View