Jitendra Pant

7 Questions 20 Answers 0 Followers

Questions related from Jitendra Pant

What is the role of reference Wavelength in cell cytotoxicity experiment?

While performing the cell cytotoxicity test, my detection wavelength is 450 nm while the reference wavelength is 650 nm. Suppose I measure my cells viability (blank, control and test) with these...

18 April 2018 1,296 5 View

What happens to the enzyme kinetics when in solution and when immobilized?

Please elaborate the change in Enzyme kinetics (v, Km etc) between an immobilized and an enzyme in solution. Thanks and regards Jitendra

19 November 2014 7,407 14 View

What could be the reason of getting a lawn of transformed cells on LB plate?

I used 20 microL of transformed DH5-a cells (pUC18 transformed, grown overnight in liquid LB after vial thawing) and spread it on LB agar (amp plate-100microL/ml) plate but was surprised to see...

03 November 2014 5,957 3 View

What is the pI of a monoclonal antibody?

I was wondering if there is a fixed pI for all monoclonal antibodies as most of the labs use glycine and phosphate buffer systems for MAb isolation using Protein A-Affinity (in HPLC).

03 November 2014 1,227 2 View

How can we make the proteins using Cell-free display, starting with mRNA directly?

Cell-free Display protein. Does it affect the protein folding?

18 September 2014 4,805 3 View

Should we use over night culture for Plasmid DNA isolation from DH5-alpha?

Hello friends, I wanted to know in which case there is a better probability (practically not theoritically) to get more plasmid yield? A) Pickup a transformed colony and put it for 12-16 hrs...

17 September 2014 2,733 7 View

What is the difference between alpha/beta and alpha +beta protein?

Please quote examples as well. Thanks in advance.

04 August 2014 1,122 6 View