How to estimate the activity of glucose 6 phoshatase and phosphoenol pyruvate carboxy kinase (PEPCK) from liver homogenite ?

03 April 2017 3,300 3 View

How to estimate serum lactate dehydrogenase (LDH) by using agappe kir method ?

I have used agapppe diagnostic kit for the estimation of serum LDH. In the procedure, 1ml of reaction mixture (4 ml of R1 + 1ml of R2) mixed with 10 micro litre of serum and incubated for 1...

08 September 2016 9,463 8 View

Can we use DMSO for dissolving corticosterone ?

for cell culture

07 August 2016 8,095 2 View

Can we use PEG for dissolving corticosterone ?

For cell culture purpose

07 August 2016 3,673 2 View

How to quantify lactate dehydrogenase in serum by biochemical method ?

reagents to be added and the calculation

06 July 2015 8,639 0 View

How to conduct antibody titre test ?

to know the level of total antibodies in the serum

01 February 2015 5,990 1 View

How do you separate cells from thymus, spleen and lymph nodes by using simple methods?

For the determination of apoptosis.

10 November 2014 5,741 2 View

How can I determine the cell growth and viability of lymphocytes by conventional method ?

to study effect of stress on lymphocytes

10 November 2014 7,291 4 View

How can I determine antioxidant status in lymphocytes ?

I need to study effect stress on lymphocytes.

10 November 2014 1,557 3 View

How to determine lipid peroxidation in lymphocytes ?

i need to study effect of stress on lymphocytes

10 November 2014 4,869 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View