Watercress supplementation in diet reduces lymphocyte DNA damage and alters blood antioxidant status in healthy adults.Lymphocytes are obtained in a fairly noninvasive way .Lymphocytes are isolated from volunteers before and after receiving a single supplement of vitamin C, vitamin E or β-carotene. The lymphocytes are treated with H2O2, and DNA strand breaks are measured by single cell gel electrophoresis (the comet assay). Significant protection against oxidative DNA damage was evident 2–4 h after vitamin C intake, and 18–24 h after consumption of the other antioxidants. Lymphocytes from smokers are found more sensitive to DNA damage than those from non-smokers, and they showed at least as great a protective effect with antioxidants.DNA damage can be assessed by the alkaline comet assay in peripheral lymphocytes, and plasma TAS levelsare determined using an automated measurement method.Peripheral lymphocyte DNA damage is caused by multiple factors including mainly oxidative stress.
Basically, you can estimate the level of antioxidant enzymes (SOD, catalase, GPX) and alternatively you can estimate oxidative damage, as lipid peroxidation, GSH/GSSG status....
Hi,
You can measure enzymes involve in this process with biochemical methods...ELISA. Real time-PCR methods.
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