How to estimate the activity of glucose 6 phoshatase and phosphoenol pyruvate carboxy kinase (PEPCK) from liver homogenite ?

03 April 2017 3,300 3 View

How to estimate serum lactate dehydrogenase (LDH) by using agappe kir method ?

I have used agapppe diagnostic kit for the estimation of serum LDH. In the procedure, 1ml of reaction mixture (4 ml of R1 + 1ml of R2) mixed with 10 micro litre of serum and incubated for 1...

08 September 2016 9,463 8 View

Can we use DMSO for dissolving corticosterone ?

for cell culture

07 August 2016 8,095 2 View

How to quantify lactate dehydrogenase in serum by biochemical method ?

reagents to be added and the calculation

06 July 2015 8,639 0 View

What is the composition of borate buffered saline ?

i need to prepare BBS of Ph 7.4

04 May 2015 7,316 1 View

How to conduct antibody titre test ?

to know the level of total antibodies in the serum

01 February 2015 5,990 1 View

How do you separate cells from thymus, spleen and lymph nodes by using simple methods?

For the determination of apoptosis.

10 November 2014 5,741 2 View

How can I determine the cell growth and viability of lymphocytes by conventional method ?

to study effect of stress on lymphocytes

10 November 2014 7,291 4 View

How can I determine antioxidant status in lymphocytes ?

I need to study effect stress on lymphocytes.

10 November 2014 1,557 3 View

How to determine lipid peroxidation in lymphocytes ?

i need to study effect of stress on lymphocytes

10 November 2014 4,869 0 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View