How to estimate the activity of glucose 6 phoshatase and phosphoenol pyruvate carboxy kinase (PEPCK) from liver homogenite ?

03 April 2017 3,300 3 View

How to estimate serum lactate dehydrogenase (LDH) by using agappe kir method ?

I have used agapppe diagnostic kit for the estimation of serum LDH. In the procedure, 1ml of reaction mixture (4 ml of R1 + 1ml of R2) mixed with 10 micro litre of serum and incubated for 1...

08 September 2016 9,463 8 View

Can we use DMSO for dissolving corticosterone ?

for cell culture

07 August 2016 8,095 2 View

Can we use PEG for dissolving corticosterone ?

For cell culture purpose

07 August 2016 3,673 2 View

How to quantify lactate dehydrogenase in serum by biochemical method ?

reagents to be added and the calculation

06 July 2015 8,639 0 View

What is the composition of borate buffered saline ?

i need to prepare BBS of Ph 7.4

04 May 2015 7,316 1 View

How do you separate cells from thymus, spleen and lymph nodes by using simple methods?

For the determination of apoptosis.

10 November 2014 5,741 2 View

How can I determine the cell growth and viability of lymphocytes by conventional method ?

to study effect of stress on lymphocytes

10 November 2014 7,291 4 View

How can I determine antioxidant status in lymphocytes ?

I need to study effect stress on lymphocytes.

10 November 2014 1,557 3 View

How to determine lipid peroxidation in lymphocytes ?

i need to study effect of stress on lymphocytes

10 November 2014 4,869 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View