How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Cross-lagged panel analysis and sample size?

I want to estimate the causal relationship between two latent variables over time using structural equation modelling. both latent variables are measured according to different indicators...

31 January 2021 3,777 5 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

SEM for panel data ?

I'm trying to find the effect of smart cities on sustainable development. I collected data for several indicators of smartness of a city for 15 cities over 12 years. seems like panel data (GMM...

12 January 2021 9,914 5 View

How to prepare H2O2 solution or certainly how can we prepare 30% w/v H2O2 solution?which solvent?

Inorder to enhance the photocatalytic activity of magnetic nano photocatalysts like zinc ferrite we can use H2O2.

10 November 2019 5,780 3 View

Does anyone know where i can purchase drug-permeable e. coli?

I need an e. coli strain that's membrane is permeable to antibiotics.

10 October 2019 2,177 3 View

No title

18 May 2019 5,751 2 View

Can anyone suggest me to set up a suitable basis set for theoretical calculations of organometallic complex?

I have recently installed Gaussian 16 for dft calculations. I have no good idea about suitable basis set? How to specify basis set for heavy atom and light atoms? YouTube tutorial mainly related...

16 February 2019 9,237 4 View

What are the tips to be considered for peptide encapsulation in liposome?

I am working on nanoliposome formulation to encapsulate a synthetic peptide , I have tried many ways for encapsulation, but after dialysis there wasn't any peptide in liposomes which I analyzed by...

02 December 2018 8,010 7 View

How do I analyse my luciferase data?

Hi I'm looking for some help in analysing my luciferase data. I have a transcription factor and then a range of proteins I think will influence transcription factor activity. For each...

08 November 2018 4,617 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View