Which factors are good for maintenance of cancer stem cells?
How can i avoid precipitation in pbs? when i solve salts separately then add to a bowel there is no precipitation but when i want to solved them in a bowel after adding 3th salts the salts...
05 June 2018 5,999 4 View
Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is...
05 June 2018 6,629 4 View
Are there companies that sell DARPins?Are there any websites that describe how to design DARPins?
09 October 2015 6,514 2 View
As you know FITC is a dim fluorochrome so I wanna know that FITC is a good fluorochrome for detection of MCF-7?Is there any problem to use of FITC for MCF-7 detection by flow? actually we treated...
31 December 2014 1,537 12 View
I'm working on CD34-FITC and used CD34- isotype control antibodies from miltenybiotec company, but the CD34-FITC control is not available in our lab right now but I have CD86-FITC isotype control...
10 November 2014 6,836 8 View
I want to detect some rare cells and I can not do analysis by FACS because of rarity and I have to detect them by fluorescence microscope but I need a protocol for this assay.
10 November 2014 3,554 13 View
I wanna know the morphology of them
02 March 2014 9,448 1 View
I have two cells and i want to know which of them express specific marker more ?
11 December 2013 9,090 3 View
I want to order some antigen and want to know which company is best?
10 November 2013 8,446 4 View
I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.
08 September 2013 8,103 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
