Why the PBS buffer precipitate?

How can i avoid precipitation in pbs? when i solve salts separately then add to a bowel there is no precipitation but when i want to solved them in a bowel after adding 3th salts the salts...

05 June 2018 5,858 4 View

Does Mesenchymal stem cell express Cardiomyocyte cells markers?

Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is...

05 June 2018 6,515 4 View

Who can give me some information about DRPINs?

09 October 2015 6,419 2 View

Has any one ever detected MCF-7 by flow cytometry with FITC-labeled antibody?

As you know FITC is a dim fluorochrome so I wanna know that FITC is a good fluorochrome for detection of MCF-7?Is there any problem to use of FITC for MCF-7 detection by flow? actually we treated...

31 December 2014 1,428 12 View

Is Isotype control antibodies (mouse IgG) for flow cytometry general?

I'm working on CD34-FITC and used CD34- isotype control antibodies from miltenybiotec company, but the CD34-FITC control is not available in our lab right now but I have CD86-FITC isotype control...

10 November 2014 6,729 8 View

How can I analyze rare cells by fluorescence microscope, not by FACS?

I want to detect some rare cells and I can not do analysis by FACS because of rarity and I have to detect them by fluorescence microscope but I need a protocol for this assay.

10 November 2014 3,439 13 View

Who can send me the photo of normal hematopoitic stem cells which isolated from cord blood?

I wanna know the morphology of them

02 March 2014 9,358 1 View

Which company produces specific antigen?

I want to order some antigen and want to know which company is best?

10 November 2013 8,346 4 View

Does anyone know which cancer cell lines (Cancer stem cell, tumor or metastatic) are positive in EpCAM?

I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.

08 September 2013 7,994 2 View

Which growth factors in culture medium can be use that are the best one for cancer stem cells or circulating tumor cells?

I want to culture CTCs and I want to make a medium that contains some growth factor that specific for CSC or CTC. Actually I want to get rid of another cells in culture medium and all the cells...

08 September 2013 4,333 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View