Fatemeh Etezadi

16 Questions 11 Answers 0 Followers

Questions related from Fatemeh Etezadi

Why the PBS buffer precipitate?

How can i avoid precipitation in pbs? when i solve salts separately then add to a bowel there is no precipitation but when i want to solved them in a bowel after adding 3th salts the salts...

06 June 2018 5,961 4 View

Does Mesenchymal stem cell express Cardiomyocyte cells markers?

Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is...

06 June 2018 6,599 4 View

Who can give me some information about DRPINs?

Are there companies that sell DARPins?Are there any websites that describe how to design DARPins?

10 October 2015 6,489 2 View

Has any one ever detected MCF-7 by flow cytometry with FITC-labeled antibody?

As you know FITC is a dim fluorochrome so I wanna know that FITC is a good fluorochrome for detection of MCF-7?Is there any problem to use of FITC for MCF-7 detection by flow? actually we treated...

01 January 2015 1,506 12 View

Is Isotype control antibodies (mouse IgG) for flow cytometry general?

I'm working on CD34-FITC and used CD34- isotype control antibodies from miltenybiotec company, but the CD34-FITC control is not available in our lab right now but I have CD86-FITC isotype control...

11 November 2014 6,800 8 View

How can I analyze rare cells by fluorescence microscope, not by FACS?

I want to detect some rare cells and I can not do analysis by FACS because of rarity and I have to detect them by fluorescence microscope but I need a protocol for this assay.

11 November 2014 3,522 13 View

Who can send me the photo of normal hematopoitic stem cells which isolated from cord blood?

I wanna know the morphology of them

03 March 2014 9,419 1 View

Is there any site for comparing the levels of expression specific marker between two cells?

I have two cells and i want to know which of them express specific marker more ?

12 December 2013 9,059 3 View

Which company produces specific antigen?

I want to order some antigen and want to know which company is best?

11 November 2013 8,413 4 View

Does anyone know which cancer cell lines (Cancer stem cell, tumor or metastatic) are positive in EpCAM?

I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.

09 September 2013 8,069 2 View

Have you ever coated cell culture plates with an antibodies for capture some cells?

If not, is it possible? How? Could you send me some article that do this approach for capture the cells in cell culture plates?

09 September 2013 8,496 4 View

What is the cause of maintenance of Circulating Tumor Cells in the blood circulation?

Which factors are good for maintenance of cancer stem cells?

09 September 2013 9,462 0 View

Can I culture isolated cells with FACS assay?

I want to isolate CTCs from whole blood with FACS. Can I culture the isolated cells?

09 September 2013 967 10 View

Which growth factors in culture medium can be use that are the best one for cancer stem cells or circulating tumor cells?

I want to culture CTCs and I want to make a medium that contains some growth factor that specific for CSC or CTC. Actually I want to get rid of another cells in culture medium and all the cells...

09 September 2013 4,419 1 View

Which stage of breast cancer patient have the highest amount of detectable CTCs in their blood?

Which group of breast cancer patients have the best sample with the highest CTCs?

08 August 2013 5,504 2 View

Does anyone have an efficient protocol for human myocardium cells isolation?

I wanna isolate human myocardium from human heart tissue for primary culture so I need an efficient and easy protocol isolation.Are there any points for this test? Does anyone have experience to...

01 January 1970 3,772 2 View