Hi i want to ask some questions about treatment of bv-2 cells with LPS. First do you prepare the LPS in serum free media? Second, how do you prepare sodium nitrite solution for calibration of NO ? Thanks
The attached method talks about using NO as an end-point after LPS stimulation. These guys simply state that they use a NO standard but I believe the original paper referenced herein will be very useful for you. (Scroll down to the bottom of this attached link for the original paper)