Dose any-one knows about cleaning validation for pathogens from fermentor?

Hi Dose any-one knows about cleaning validation protocols for pathogen-bacteria from fermentor? In industerial size, 1000 lit?

15 December 2020 4,418 3 View

How can we calculate the concentration of 1000 tons of carbon dioxide in the air in PPM?

Dear All scientists; How can we calculate the concentration of CO2 emitted in the air in PPM? shall we use PPMv(Volume PPM) or Mass PPM is also acceptable? Regards

01 August 2019 7,464 4 View

How can I recall a modified citation style in Mendeley with the name I entered?

When I save as a modified style and change its name, the new style does not show up with the name I have entered for in the styles list. In stead, a general title like "APA-Saeideh" is built...

20 May 2019 6,622 6 View

Can I run GEE having two within factor variables?

I have one factor as time (3 time points) and the other as treatment( absorbable versus nonabsorbable Sutures that each of them were used on different sides of a scar) so two treatments had been...

08 June 2018 6,220 1 View

How can I make a teeth whitener in the chemistry lab?

How can I make a teeth whitener in the chemistry lab?

02 June 2018 6,306 3 View

Could anyone suggest a good way to dissolve lard for making a high fat diet?

I am trying to make a ketogenic diet using lard and wonder how to dissolve lard and have a solidify mix of carbohydrate+protein+ lard at the end for 1 litre?

29 May 2017 7,074 3 View

Any advice on symmetry solvents for freezing polymer blends?

Why high symmetry solvents are proper for freez polymer blends?

29 December 2015 3,684 3 View

Does anyone have any idea how to inhibit liquid loss inside the capillary tube?

I have two chambers, the inner contain a capillary tube and the outer chamber contains water to maintain the humidity. My problem is that when I fill the capillary tube (by capillary action) and I...

16 September 2015 1,293 7 View

No title

19 January 2015 4,924 7 View

Do you know any evaporation retardant for PMA (2-methoxy-1-methylethyl acetate)?

12 January 2015 7,399 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View