Dose any-one knows about cleaning validation for pathogens from fermentor?

Hi Dose any-one knows about cleaning validation protocols for pathogen-bacteria from fermentor? In industerial size, 1000 lit?

15 December 2020 4,418 3 View

How can we calculate the concentration of 1000 tons of carbon dioxide in the air in PPM?

Dear All scientists; How can we calculate the concentration of CO2 emitted in the air in PPM? shall we use PPMv(Volume PPM) or Mass PPM is also acceptable? Regards

01 August 2019 7,464 4 View

How can I recall a modified citation style in Mendeley with the name I entered?

When I save as a modified style and change its name, the new style does not show up with the name I have entered for in the styles list. In stead, a general title like "APA-Saeideh" is built...

20 May 2019 6,622 6 View

Can I run GEE having two within factor variables?

I have one factor as time (3 time points) and the other as treatment( absorbable versus nonabsorbable Sutures that each of them were used on different sides of a scar) so two treatments had been...

08 June 2018 6,220 1 View

How can I make a teeth whitener in the chemistry lab?

How can I make a teeth whitener in the chemistry lab?

02 June 2018 6,306 3 View

Any advice on symmetry solvents for freezing polymer blends?

Why high symmetry solvents are proper for freez polymer blends?

29 December 2015 3,684 3 View

Does anyone have any idea how to inhibit liquid loss inside the capillary tube?

I have two chambers, the inner contain a capillary tube and the outer chamber contains water to maintain the humidity. My problem is that when I fill the capillary tube (by capillary action) and I...

16 September 2015 1,293 7 View

What is the best way to improve the yield of exosome extracted miRNA for NGS?

I extract miRNA from serum's exosomes and the yield of RNA is low for downstream experiments, Next Generation Sequencing. I have tried different protocols, Exoquick, UC, miRNeasy Qiagen, Nerogen,...

06 May 2015 548 2 View

No title

19 January 2015 4,924 7 View

Do you know any evaporation retardant for PMA (2-methoxy-1-methylethyl acetate)?

12 January 2015 7,399 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

No title

22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View