Greetings to all,
I would like to extract as many lipid components as possible from a vegetable oil. In principle, all polar lipids and as many neutral lipids as possible.
But I need to test this extract in cell culture. So I am limited in my choice of solvent. I think the main solvent component should be the DMSO.
The question is whether Tween 80 can help with the extraction? Will a small amount of ethanol and/or ethanolamine increase the efficiency?
Which is the better approach: pure DMSO (plus other putative components) or water/DMSO mixture?
NB. I don't need to emulsify, I would like to create something as close as possible to the true solution.
Thank you for this very helpful detailed answer. I am wondering if isopropyl alcohol is compatible with physiological cell culture media. These media contain many different salts.
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