Lëonid Volkov

13 Questions 40 Answers 0 Followers

Questions related from Lëonid Volkov

Could someone suggest a company working in the field of artificial meat (cellular agriculture)?

Hi, I am looking for a company operating in the field of artificial meat. By operating, I mean production or advanced stage of pre-production. I am looking for because we have some good solutions...

25 November 2022 7,340 8 View

Could anyone suggest a source of water soluble oligomers of polylactide?

I have seen in the literature that short chain poly-lactic acid (polylactide; PLA) oligomers are water soluble. But I am unable to find a commercial source of this type of oligomers. Does anyone...

01 November 2022 1,499 6 View

Can anyone suggest a mercury-free LED light source for fluorescence microscopy?

Can anyone suggest a mercury-free LED light source for fluorescence microscopy? I'm looking for the simplest and most economical replacement for mercury bulbs. Not the complicated stuff with many...

06 October 2022 6,137 3 View

Could isopropyl alcohol (2-propanol) be used instead of DMSO for the stock solutions for cell cultures?

For various reasons, I need to avoid DMSO with my cells. So I wonder if isopropyl alcohol (2-propanol) could be used instead of DMSO to create my stock solutions library? I have learned that the...

05 July 2022 3,736 1 View

What are the possible reasons for fibroblastic transformation of confluent CHO-K1?

Hello to all I observed a strange phenomenon with CHO-K1 following the change of serum batch (it is surely the toxicity of this batch but I am very curious about the possible nature of this...

22 April 2022 6,233 3 View

What is the best way to extract polar lipids from a vegetable oil?

Greetings to all, I would like to extract as many lipid components as possible from a vegetable oil. In principle, all polar lipids and as many neutral lipids as possible. But I need to test this...

26 March 2021 8,824 2 View

Materno-foetal exchanges of amino acids during gestation ?

I study amino acid transport through (and synthesis by) placenta in different species. I found the data about paired dam-fetus serum (plasma) content of AA in horse, sheep, pig etc but not in cow....

23 April 2019 5,064 1 View

How to sterilize a highly viscous homogenate by filtration?

Hello everyone! We have a highly viscous homogenate. It looks like a juice containing pulp, but we need to cold(!)-sterilize it. Of course, any "pulp" must be eliminated and only the "juice" will...

04 August 2018 570 10 View

What is the best mounting medium for the STED with fixed cells?

Leica and Abberior say: ¨Please do not use Vectashield, Vectashield Hard set or (other) embedding media containing p-phenylenediamine as antifading reagent." (I do not understand why, but I have...

01 February 2018 2,595 7 View

Does anyone know a psychrophilic pathogen strain producing a lot of proteases ?

It could be for exemple an "analog" of Clostridium histolyticum causing gangrene-like diseases in cold-water fish.thank at advance

09 April 2016 7,559 0 View

Can someone, please, suggest a fluorescent scanner with the light source placed above the sample?

I have to scan a non-transparent petri dish sized (in term of diameter and thickness) object. The colonies of fluorescent bacteria will be on its upper side. Thus, the UV excitation must come from...

08 April 2016 4,193 7 View

What is notch 1 lethal silent mutation?

Good day, I found by the Google this phrase in the scientific discussion: Mice homozygous for the recessive Notch 1 mutation die as embryos. When the nucleotide sequences of the wild-type and...

13 March 2015 8,385 2 View

Could anyone suggest a maker of good metallic nanoparticles ready for conjugation with antibodies?

“Good” means: non-aggregating, non-precipitating, stable … etc “ready for conjugation” means that we have just to add antibodies, wait X minutes, then centrifuge, resuspend in PBS and use. Thanks.

29 May 2014 7,724 1 View