could anyone explain me what is the best way to do a microdilution antimicrobial assay: incubating a ELISA microplate in standard incubator for 24h and read just initial and final absorbance, or using an ELISA plate reader for incubate and read absorbances every hour ,for 24 hours.
incubating a ELISA microplate in standard incubator for 18-24h and read just initial and final absorbance was best as use control positive and control negative wells.
The attached publication uses a micro dilution method to determine MIC and may be helpful.
Article Interactive antimicrobial and toxicity profiles of conventio...
Hi Paula
MICs are normally determined after 16-20 hours by visual inspection of the microtiter plate. I would recommend that you adhere to an approved standard for susceptibility testing, such as that issues by the CLSI. If you plan to use breakpoints to determine resistance/susceptibility (i.e. not only recording the MIC), then the methodology used in MIC testing should be according to the methodology recommended by the breakpoint-setting organisation. Good luck!
The lecture should be made within incubation 18-24 hours for most of the pathogenic bacteria. You must be care with controls, they are very important in order to withdraw appropriated results
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