best temperature to store protein extraction buffer containing Tris, EDTA, NaCl and tween-20
You can store your buffer at room temperature, or even at 4 degree, just as sugested by Feng Lin. Bear in mind that when using Tris as a buffering component, the pH of your buffer will be different when stored at 4 degree or RT. Since pH plays a crucial role in maintaining the stability of proteins, therefore, it is important for you to measure the pH accordingly, when storing the buffer either at 4 degree or at RT.
You may want to refer to this site for more information:
http://www.sigmaaldrich.com/Graphics/Supelco/objects/4800/4709.pdf
Hope this helps :-)
I agree, 4 degrees is the way to go. I'm assuming you keep your samples on ice when doing the extraction, so it only makes sense to use pre-chilled extraction buffer.
thank you Saranpal and Jordan for your kind reply.
yes I always keep my samples on ice
The storage buffer(without the protein) can be stored at room temperature. But the point is when you want to use it gain after a while you have to re add PMSF and Benzenimide again since they go bad after a while.
The best suitable temperature to store protein in buffer is 4 Degree Celsius. But, you can store buffer at room temperature (Only Extraction Buffer without Protein).
Dear Pratima,
what kind of samples do you use with this buffer? And how do you calculate the concentration of the protein?
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