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Questions related from Pratima Chawla
is there any problem with the GV3101 competent cells of mine. I made stock for Agro GV3101 and check its sensitivity and resistance to certain antibiotics. they are growing just in overnight...
15 May 2015 7,438 6 View
best temperature to store protein extraction buffer containing Tris, EDTA, NaCl and tween-20
13 May 2015 5,356 16 View
I extracted total protein from agro-infiltered N. benthamiana leaves by using following extraction buffer :- Protein Extraction Buffer- 50mM Tris/HCl pH 7.6, 100mM NaCl, 0.05% Tween20, 1mM EDTA,...
05 March 2015 3,271 5 View
I want to do confocal microscopy of Nicotiana benthamiana in filtered leaves but I want to prepare permanent slides for that which can be stored. Basically first I want to observe my samples in...
02 December 2014 3,875 5 View
Please can anyone suggest a good book on Plant (Arabidopsis) response to stress which also includes UPR and other signalling pathways? Or collective data in a graphical form or animated form....
21 November 2014 8,118 3 View
I know the sequence of my gene but want to do the RACE to confirm the position of 5 end prime and 3' end prime. Now, I am confused about how RACE works in this case and we can say by seeing at...
30 July 2014 5,525 3 View
How should I make primers to check that? I want to design primers to check the T-DNA insertion mutant line, want to learn from scratch.
23 October 2013 3,024 3 View