Feng Lin

10 Questions 18 Answers 0 Followers

Questions related from Feng Lin

Why is the localization of protein in vivo is different from that of fixation?

Hi folks, I am recently working with Arabidopsis to look at the protein which has been reported to be at cell plate involved in cytokinesis with YFP tag. However, when I fixed seedlings which...

12 March 2017 379 3 View

Why are there no visible layers in liposome flotation?

Hi guys, I did liposome flotation. Ithe liposome and protein were mixed in 30% sucrose(about 333ul), then overlaid with 200ul 25% sucrose, followed by adding 200ul HEPES buffer on top, then...

13 October 2016 2,386 2 View

A good anti c-myc antibody?

Hi guys, I recently used anti c-myc antibody purchased from Sigma (C3956) to do whole mount immunostaining. However, the signal appered somtimes. In most cases, no signal. Does anybody know this?...

19 July 2016 4,563 4 View

Dose anybody encounter the problem of mutaion in wide-type Arabidopsis (ecotype: Columbia 0)?

Hi guys, recently I encounter a big problem. I cloned my gene from whole genomic DNAof wide-type (Col-0), and I found that there are some mutations in bases, I have tried this two times and...

23 June 2015 392 6 View

How do genotype my T-DNA insertion Arabidopsis line?

Hi everybody, I now encounter a overwhelming problem about genotyping. after first round genotyping, I selected the positive seedlings,i.e., with FP+RP, no wide type DNA was amplified, with LB+RP,...

22 May 2015 2,090 9 View

How do I measuring microtubule density in Arabidopsis with image J?

Hi guys, I want to measure microtubule density with image J, but I don't know how to do this. Is there anybody being familiar with method? Although there is a method to measure density by counting...

20 May 2015 1,328 6 View

Can anyone help with floral dipping of Arabidopsis?

Hi guys, I am recently doing arabidopsis transformation by floral dipping. However, after dipping, the flowers were dead. Does anybody know what happened? Here is my protocol: 5% sucrose, 0.05%...

04 May 2015 972 5 View

Any suggestions on the attempted GST tagged protein expression?

Hi guys, I am encountering a problem that my protein can't be purified by affinity purification, namely GST resin. This protein is a mutated protein where 20 amino acids were deleted. My protocol...

28 April 2015 7,187 6 View

How can I maintian RFP fluorescence?

Can RFP fluorescence still be kept after paraformaldehyde fixtation? I recently wanted to do a IF-ststainig, and as far as I know, GFP fluorescence can be well preserved after paraformaldehyde...

23 March 2015 203 3 View

Any suggestions about immunostaining microtubules of root tip in Arabidopsis ?

Dear all, recently, I am immunostaining mitotic microtubule arrays of root tips in Arabidopsis. I find that the tissues alwas collapse after immunostaining, and sometimes, only epidermal and...

15 February 2015 2,315 5 View