11 Questions 19 Answers 0 Followers
Questions related from Feng Lin
Hi folks, I am recently working with Arabidopsis to look at the protein which has been reported to be at cell plate involved in cytokinesis with YFP tag. However, when I fixed seedlings which...
12 March 2017 383 3 View
Hi guys, I did liposome flotation. Ithe liposome and protein were mixed in 30% sucrose(about 333ul), then overlaid with 200ul 25% sucrose, followed by adding 200ul HEPES buffer on top, then...
13 October 2016 2,391 2 View
Hi guys, I recently used anti c-myc antibody purchased from Sigma (C3956) to do whole mount immunostaining. However, the signal appered somtimes. In most cases, no signal. Does anybody know this?...
19 July 2016 4,568 4 View
Hi guys, recently I encounter a big problem. I cloned my gene from whole genomic DNAof wide-type (Col-0), and I found that there are some mutations in bases, I have tried this two times and...
23 June 2015 397 6 View
Hi everybody, I now encounter a overwhelming problem about genotyping. after first round genotyping, I selected the positive seedlings,i.e., with FP+RP, no wide type DNA was amplified, with LB+RP,...
22 May 2015 2,095 9 View
Hi guys, I want to measure microtubule density with image J, but I don't know how to do this. Is there anybody being familiar with method? Although there is a method to measure density by counting...
20 May 2015 1,332 6 View
Hi guys, I am recently doing arabidopsis transformation by floral dipping. However, after dipping, the flowers were dead. Does anybody know what happened? Here is my protocol: 5% sucrose, 0.05%...
04 May 2015 977 5 View
Hi guys, I am encountering a problem that my protein can't be purified by affinity purification, namely GST resin. This protein is a mutated protein where 20 amino acids were deleted. My protocol...
28 April 2015 7,191 6 View
Can RFP fluorescence still be kept after paraformaldehyde fixtation? I recently wanted to do a IF-ststainig, and as far as I know, GFP fluorescence can be well preserved after paraformaldehyde...
23 March 2015 207 3 View
Hi everybody, recently, I encounter a problem of sample drifting under confocal microscope. As I want to record mitosis and cytokinesis which take 1 hour in total in arabidopsis roots, but...
09 March 2015 2,388 2 View
Dear all, recently, I am immunostaining mitotic microtubule arrays of root tips in Arabidopsis. I find that the tissues alwas collapse after immunostaining, and sometimes, only epidermal and...
15 February 2015 2,319 5 View
