Teresa Gallastegi Mozos

4 Questions 12 Answers 0 Followers

Questions related from Teresa Gallastegi Mozos

Any suggested recombinant human tgfB1?

Hello, I have been facing some troubles with recombinant human tgfB1 cytokine from peprotech. I use it in order to induce a contractile phenotype to human vascular smooth muscle cells. Is there...

25 January 2019 9,985 4 View

Does anybody work with aorta tissue samples and have an optimized method to extract proteins from it?

I have tried to perform protein extraction from aorta samples but whenever I perform an SDS-PAGE with those samples I obtained different results of the same protein. It seems as if the protein...

04 October 2016 5,150 17 View

Has anybody used Alexa Fluor 647 Mouse Anti-Human CD66b antibody for inmunofluorescence staining?

Hi all, The antibody Alexa Fluor 647 Mouse Anti-Human CD66b has been tested for flow cytometry but I would like to know if anybody has also tried it for inmunofluorescence and whether it was...

26 February 2016 8,701 1 View

Is there anybody who has already tried to transfect a cell line with the Control double Nickase plasmid from santa cruz technologies?

I have tried to transfect 2 different cell lines with this plasmid but got no success. First of all, I transfect HEK 293 cell line with its transfection reagent and although I could see cell had...

10 November 2015 933 2 View