Saranpal Singh

4 Questions 21 Answers 0 Followers

Questions related from Saranpal Singh

How do I interpret the results for CD Spectroscopy for my protein?

Hi, I have got CD results for my protein. However I do not know how to interpret the data for CD. I have uploaded my results in word format. Kindly download to take a look at the results. I...

23 June 2015 2,087 9 View

Can sarkosyl be used for the solubilization of inclusion Bodies?

Dear Esteemed Researchers, I am trying to solubilize a membrane protein that forms inclusion body when expressed heterologously in E. coli cells. As such, I tried various detergents to screen the...

01 May 2015 9,492 13 View

Can I perform electroporation on Pichia using 0.1cm cuvette? Has anyone tried this?

I will be performing electroporation on Pichia to express a recombinant protein. Is it possible to use 0.1cm cuvette (Frequently used for E.coli or other bacteria) rather than 0.2 cm cuvette? Will...

28 December 2013 2,972 8 View

Cleaving NusA tagged Fusion protein?

I have cloned my gene downstream of the NusA tag using BamHI and HindIII as my restriction sites. Now that my protein is expressing, I would like to cleave this tag and purify the protein to...

03 November 2013 8,965 1 View