XRF analysis of 45s5 analysis?

I've sent my synthesized 45s5 powder and a commercial 45s5 powder to XRF analysis but in both of them the amount of Na2O is about 15 percent and LOI is around 40 percent and it makes the aount of...

29 January 2021 239 4 View

Sol-gel synthesis of 45S5?

I've synthesized 45S5 bioglass by sol-gel method and my XRD and FTIR results are similar to 45S5 bioglass but my EDX result has differences and instead of Si and Na the amount of Ca is higher....

29 December 2020 4,847 2 View

Sol-gel Synthesis of hydroxyapatite?

I use Ca(NO3)2.4H2O and P2O5 as precursors and Sol-gel method to synthesis my powder but the CA/P ratio is more than stochiometric (167) and my powder is dark. Does anyone know why this happens?

27 December 2020 1,574 5 View

Could anyone help me with this error Exception has occurred: ValueError?

I have downloaded monthly mean wind speed of terraclimate data for 20 years (2000-2019) which have NetCDF format, but unfortunately, each year has a single NetCDF file, and I want to merge them...

25 November 2020 8,477 2 View

Has anyone used Breaks For Additive Season and Trend for decomposing the time series into trend,and season in R package?

12 September 2020 1,513 3 View

How show NDVI MODIS index (MOD13A1) in Arc GIS?This collection of MODIS has calculated NDVI index and i want to show this index in my study area?

This collection of MODIS has 2 options for calculating NDVI index. First, we can calculate it from NDVI formula or we can use NDVI index that MODIS has calculated. I use both of them but they are...

25 August 2020 2,419 3 View

Has anyone used Maximum likelihood or Support vector machine (SVM) for image classification?

Hello I'm working on a project that I want to detect forest and deforestation areas. I need some information about Maximum-likelihood or Support vector machine (SVM) for image classification.

22 August 2020 1,896 3 View

How we can use the Transmisivity of light colors (rtn, gtn, and btn) in simulating a coated glass with red dyes in Radiance?

We want to simulate a red coated glass in Radiance. The UV-VIS spectroscopy analysis was conducted and optical characteristics of the red glass including transmittance and absorbance were...

16 March 2020 3,492 5 View

Model game as a discrete-time Markov chain?

Model game as a discrete-time Markov chain

30 December 2019 5,346 3 View

How can i remove fat from alkaloids?

sometimes i have problem with fat in my extracted alkaloids using ethanol and chloroform for extraction. Also my alkaloids are not well dissolved in acidic water, so acid-base precipitation...

09 December 2018 2,737 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View