Hello everyone
I am working on a human cell-line of Oligodendrocyte Precursor cell (OPCs) called MHB. Results of my immunocytochemistry experiments showed that these cells express NG2, and don’t express CNPase or MBP, confirming their progenitor stage. I am trying to mature these cells to myelin producing oligodendrocytes expressing MBP, MOG, GalC; but most of the publications focus on the primary OPCs, and I couldn’t find any protocols to make OPCs cell-lines mature. If you are working in this field or have an experience on this, I would truly appreciate your comments and guidance.