What is the best protocol for DNA extraction of bovine papiloma warts?

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Where i can get the software ASSIGN 400ATF?

Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?

10 November 2017 401 0 View

Does the primer used in a PCR may differ in bp size in electrophoresis?

We are performed an electrophoresis with primers taken from a paper based on hemiparasites samples. The posterior electrophoresis was different from the original work 160bp vs 700bp in several...

09 October 2015 7,977 7 View

Can someone know about Virotype data interpretation?

Can someone help where can find the data sheet of Qiagen virotype BTV RT-PCR kit serotypes 1 to 24 chart CT's

05 June 2015 7,731 2 View

Any suggestions for the Rotor gene troubleshooting?

A RT PCR rotor gene was used for blue tongue diagnosis and the curve was absent either the internal control was absent, any suggestions?

01 February 2015 9,453 3 View

What is the best source for RNA extraction: Blood or Serum in Blue tongue?

In a RT-PCR for diagnosis of blue tongue virus what is the best source of viral RNA blood or serum

31 December 2013 5,145 0 View

Can someone recommend a software to analize the poliploid data from microsatellites in plants?

The actual softwares like population, Genepop, Genalex, Genetix, Arlequín, etc, works with diploid data but we have a many aleles in one locus

06 July 2013 871 4 View

Can I use PBS-TWEEN instead PBS in Histopaque 1077 to dilute blood for isolation of mononuclear cells?

I am going to make an DNA extraction from mononuclear cells and the Histopaque 1077 protocol use PBS but I have PBS-TEEN

06 July 2013 870 6 View

About Fastq and Fatsa file?

Can someone help me to find a software convert fastq to fasta archive?

01 January 1970 872 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View