In a RT-PCR for diagnosis of blue tongue virus what is the best source of viral RNA blood or serum
We are trying to extract viral DNA from warts of bovine to diagnose papilloma virus disease but. Can someone help us to find the best way?
01 February 2018 1,451 2 View
Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?
10 November 2017 250 0 View
We are performed an electrophoresis with primers taken from a paper based on hemiparasites samples. The posterior electrophoresis was different from the original work 160bp vs 700bp in several...
09 October 2015 7,844 7 View
Can someone help where can find the data sheet of Qiagen virotype BTV RT-PCR kit serotypes 1 to 24 chart CT's
05 June 2015 7,558 2 View
A RT PCR rotor gene was used for blue tongue diagnosis and the curve was absent either the internal control was absent, any suggestions?
01 February 2015 9,322 3 View
06 July 2013 760 4 View
I am going to make an DNA extraction from mononuclear cells and the Histopaque 1077 protocol use PBS but I have PBS-TEEN
06 July 2013 759 6 View
Can someone help me to find a software convert fastq to fasta archive?
01 January 1970 732 2 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View