What is the best protocol for DNA extraction of bovine papiloma warts?

We are trying to extract viral DNA from warts of bovine to diagnose papilloma virus disease but. Can someone help us to find the best way?

01 February 2018 1,451 2 View

Where i can get the software ASSIGN 400ATF?

Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?

10 November 2017 250 0 View

Does the primer used in a PCR may differ in bp size in electrophoresis?

We are performed an electrophoresis with primers taken from a paper based on hemiparasites samples. The posterior electrophoresis was different from the original work 160bp vs 700bp in several...

09 October 2015 7,844 7 View

Can someone know about Virotype data interpretation?

Can someone help where can find the data sheet of Qiagen virotype BTV RT-PCR kit serotypes 1 to 24 chart CT's

05 June 2015 7,558 2 View

Any suggestions for the Rotor gene troubleshooting?

A RT PCR rotor gene was used for blue tongue diagnosis and the curve was absent either the internal control was absent, any suggestions?

01 February 2015 9,322 3 View

What is the best source for RNA extraction: Blood or Serum in Blue tongue?

In a RT-PCR for diagnosis of blue tongue virus what is the best source of viral RNA blood or serum

31 December 2013 5,010 0 View

Can I use PBS-TWEEN instead PBS in Histopaque 1077 to dilute blood for isolation of mononuclear cells?

I am going to make an DNA extraction from mononuclear cells and the Histopaque 1077 protocol use PBS but I have PBS-TEEN

06 July 2013 759 6 View

About Fastq and Fatsa file?

Can someone help me to find a software convert fastq to fasta archive?

01 January 1970 732 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View