What is the best protocol for DNA extraction of bovine papiloma warts?

We are trying to extract viral DNA from warts of bovine to diagnose papilloma virus disease but. Can someone help us to find the best way?

01 February 2018 1,451 2 View

Where i can get the software ASSIGN 400ATF?

Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?

10 November 2017 250 0 View

Does the primer used in a PCR may differ in bp size in electrophoresis?

We are performed an electrophoresis with primers taken from a paper based on hemiparasites samples. The posterior electrophoresis was different from the original work 160bp vs 700bp in several...

09 October 2015 7,844 7 View

Can someone know about Virotype data interpretation?

Can someone help where can find the data sheet of Qiagen virotype BTV RT-PCR kit serotypes 1 to 24 chart CT's

05 June 2015 7,558 2 View

What is the best source for RNA extraction: Blood or Serum in Blue tongue?

In a RT-PCR for diagnosis of blue tongue virus what is the best source of viral RNA blood or serum

31 December 2013 5,010 0 View

Can someone recommend a software to analize the poliploid data from microsatellites in plants?

06 July 2013 760 4 View

Can I use PBS-TWEEN instead PBS in Histopaque 1077 to dilute blood for isolation of mononuclear cells?

I am going to make an DNA extraction from mononuclear cells and the Histopaque 1077 protocol use PBS but I have PBS-TEEN

06 July 2013 759 6 View

About Fastq and Fatsa file?

Can someone help me to find a software convert fastq to fasta archive?

01 January 1970 732 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View