I am going to make an DNA extraction from mononuclear cells and the Histopaque 1077 protocol use PBS but I have PBS-TEEN
I wouldn't do it. The detergent can lyse the cells and ruin your experiment.
Thanks Jason
Can i use just the Histopaque 1077 with blood?
To separate the buffy coat
Yes, 1077 will work.
I wouldn't use tween in the PBS, the detergent will damage your cells. You can use 1077
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