Can I use PBS-TWEEN instead PBS in Histopaque 1077 to dilute blood for isolation of mononuclear cells?

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Jason Rosenzweig

I wouldn't do it. The detergent can lyse the cells and ruin your experiment.

Axel Villalobos Cortés

Thanks Jason

Can i use just the Histopaque 1077 with blood?

To separate the buffy coat

Yes, 1077 will work.

Natalie Norton

I wouldn't use tween in the PBS, the detergent will damage your cells. You can use 1077