What is the best protocol for DNA extraction of bovine papiloma warts?

We are trying to extract viral DNA from warts of bovine to diagnose papilloma virus disease but. Can someone help us to find the best way?

01 February 2018 1,451 2 View

Where i can get the software ASSIGN 400ATF?

Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?

10 November 2017 250 0 View

Does the primer used in a PCR may differ in bp size in electrophoresis?

We are performed an electrophoresis with primers taken from a paper based on hemiparasites samples. The posterior electrophoresis was different from the original work 160bp vs 700bp in several...

09 October 2015 7,844 7 View

Can someone know about Virotype data interpretation?

Can someone help where can find the data sheet of Qiagen virotype BTV RT-PCR kit serotypes 1 to 24 chart CT's

05 June 2015 7,558 2 View

Any suggestions for the Rotor gene troubleshooting?

A RT PCR rotor gene was used for blue tongue diagnosis and the curve was absent either the internal control was absent, any suggestions?

01 February 2015 9,322 3 View

What is the best source for RNA extraction: Blood or Serum in Blue tongue?

In a RT-PCR for diagnosis of blue tongue virus what is the best source of viral RNA blood or serum

31 December 2013 5,010 0 View

Can someone recommend a software to analize the poliploid data from microsatellites in plants?

06 July 2013 760 4 View

Can I use PBS-TWEEN instead PBS in Histopaque 1077 to dilute blood for isolation of mononuclear cells?

I am going to make an DNA extraction from mononuclear cells and the Histopaque 1077 protocol use PBS but I have PBS-TEEN

06 July 2013 759 6 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View