I want to normalize the data I obtain from 96 well plate and I planned to normalize the data on the basis of cell number. Can you suggest me the methods which I can use to count my cells ? I will prefer Non-fluorescence based methods to do that.
Rahul Mallick
I prefer to count in serpentine way. Start counting the cells from left upper well and go ahead. And it's better to have a counter in your free hand. So, you don't need to move out your eyes from the microscope.
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Daniil R. Petrenyov
Colometric SRB is common.
Colometric MTT is possible sometimes.
Fluorescent DNA is optional.
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Arindam Dey
Thanks Rahul for your answer but I think counting 96 wells manually will be too hard and it could also affct the accuracy.
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