Rahul Mallick

4 Questions 8 Answers 0 Followers

Questions related from Rahul Mallick

What could be the best way to select candidate genes related to a disease and how to validate them?

I am working on network medicine of Alzheimer's disease. To see all possible physical interactions in PPI network, I need to figure out AD risk genes. I found various no. of genes on various...

03 March 2018 4,338 0 View

How to produce a coding sequence of gene of interest?

I got the sequence of my gene of interest (FGF2) from NCBI clone library. I want to produce that gene and I shall transfer that gene into a plasmid. What is the best option?

07 July 2017 1,155 0 View

How can I get best ligation and transformation?

I have 2 plasmids. I have digested 1 plasmid(vector) with Sal1 and BamH1 (size 7.8kb) & other one(insert) with EcoR1 and BamH1 (size 5.3kb). I have blunted both vector and insert &...

03 March 2017 719 0 View

Why I can't see any DNA band on Agarose gel?

I have transformed my cloned plasmid DNA into bacteria and cultured bacteria. Then, I extracted DNA from bacteria and run in 0.5% agarose gel. But I am not seeing any DNA band except ladder. (In...

02 February 2017 764 13 View