I am observing a band in my western blots from a test sample which is actually devoid of it. However, my control sample contains it. Hence, I am suspecting that some sort of cross-contamination might have occurred during protein lysate preparation using the homogenizer. hence, I would like to the effective ways to completely clean a homogenizer before switching sample. Any suggestions?
Homogenize a detergent solution. In future, homogenize the negative control first.
That depends on the time you will have for homogenization. If you want it to be effective, rinse or homogenize with clean water first, routine bleach and with detergent to clean out the bleach, then with again water. This looks tedious but effective in cleaning out any carrying cross contaminants.
Adam and Lakshmi Narayana,
Thank you for the suggestions. I clean the homogenizer with 70% ethanol and water. Never tried the option of bleach. Can you also please mention about the % of bleach and detergent to be used for the same?
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