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Questions related from Wulligundam Praveen
http://servers.binf.ku.dk/bloodspot/ The website addressed to the above link goes inaccessible at times. The reasons are unknown. This is a key database accessed by many hematopoiesis...
16 March 2021 8,076 3 View
Flow cytometry data which can be presented in various ways like dot plots, contour, density plots, etc. But in all these representations, the depth of a given population is not very clear in 2D...
10 November 2018 9,905 3 View
I am curious to know about the effects of fixation, if any, esp. by paraformaldehyde (2-4%) on cell surface markers (and also intra-cellular proteins) and subsquent flow cytometry analysis of...
21 October 2017 5,288 3 View
pH value of blood is between 7.35 - 7.45. Yet, it is generally practiced to use EDTA solutions of various concentrations (as per need) at a pH value of 8.0. If this relatively higher pH of 8.0 is...
26 August 2017 6,171 6 View
I want to show the data points about the test and control samples in a vertical dot plot/scatter plot using Excel 2016. Have tried in various ways to figure it out, but could not. Can anyone...
09 August 2016 853 7 View
I currently use OP9 cells for hematopoietic differentiation of mESCs, which is taking a lot of time. Multiple times, the OP9-mESC co-cultures go bad and show an altered morphology and spatial...
25 December 2015 9,770 0 View
After completing an experiment I've found that the medium used for differentiating mESCs (spontaneous) was with excess of L-Glutamine content. In what way does this affect the spontaneous...
03 December 2014 4,025 6 View
At the first instance I was surprised to know that B cell development is different and independent (?) of B cells/progenitors from bone marrow. It is also shown that the thymic B cells are...
01 January 1970 8,657 0 View
