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Questions related from Wulligundam Praveen
http://servers.binf.ku.dk/bloodspot/ The website addressed to the above link goes inaccessible at times. The reasons are unknown. This is a key database accessed by many hematopoiesis...
16 March 2021 8,116 3 View
Flow cytometry data which can be presented in various ways like dot plots, contour, density plots, etc. But in all these representations, the depth of a given population is not very clear in 2D...
10 November 2018 9,947 3 View
I generally use GAPDH, alpha-tubulin, beta-tubulin and vinculin as loading control for my western blotting experiments and gapdh for qPCR analysis. But recent publications show that in the...
07 November 2018 4,932 3 View
Hi all. I came across a technical question from one of my friends, that whether a given culture of hESCs can be inserted with three different reporter constructs using CRISPR/Cas9 method, which...
11 April 2018 850 4 View
I would like to tag a few proteins which are in the range of 15 kDa to 35 kDa and use them for cell-culture based experiments. GFP and its derivatives being bulky, are not an option for me, at...
17 February 2018 2,372 11 View
I am curious to know about the effects of fixation, if any, esp. by paraformaldehyde (2-4%) on cell surface markers (and also intra-cellular proteins) and subsquent flow cytometry analysis of...
21 October 2017 5,322 3 View
pH value of blood is between 7.35 - 7.45. Yet, it is generally practiced to use EDTA solutions of various concentrations (as per need) at a pH value of 8.0. If this relatively higher pH of 8.0 is...
26 August 2017 6,207 6 View
I am observing a band in my western blots from a test sample which is actually devoid of it. However, my control sample contains it. Hence, I am suspecting that some sort of cross-contamination...
23 January 2017 7,893 3 View
I want to show the data points about the test and control samples in a vertical dot plot/scatter plot using Excel 2016. Have tried in various ways to figure it out, but could not. Can anyone...
09 August 2016 903 7 View
I currently use OP9 cells for hematopoietic differentiation of mESCs, which is taking a lot of time. Multiple times, the OP9-mESC co-cultures go bad and show an altered morphology and spatial...
25 December 2015 9,802 0 View
I would like to know the possible upper limit of no. of passages for pluripotent stem cells.
21 July 2015 2,072 4 View
In literature, there is a difference in composition of differentiation medium used for mES/OP9 co-culture method. Some have used with beta-mercaptoethanol (beta-ME) where as some did not. I would...
18 April 2015 4,459 2 View
After completing an experiment I've found that the medium used for differentiating mESCs (spontaneous) was with excess of L-Glutamine content. In what way does this affect the spontaneous...
03 December 2014 4,063 6 View
At the first instance I was surprised to know that B cell development is different and independent (?) of B cells/progenitors from bone marrow. It is also shown that the thymic B cells are...
01 January 1970 8,692 0 View
