What is the best method to analyse EBV?

More Mona Ellaithi's questions See All

Do you know journals for free publication cost?

Dear all, i am looking for a list or suggestions for biomdical jurnals that can publish for free or waiver publication cost for authors from low income countries. I know about the BMCs but I need...

04 May 2019 8,944 4 View

How to use NEBcutter?

Hello everyone, I would like to use RFLP to detect specific SNP. I would like to selelect specific enzyme that cuts in certain nucleaotide using NEBcutter but I do not know if I did well using...

10 November 2018 5,808 0 View

What are the parameter of the bioinformatic tools?

Dear all, Now a days there are too many bioinformatic tools and sftwares freely available on the net like SIFT and Polyphen. These tools are able to predict the effect of specific varaiant on...

09 October 2018 732 2 View

Is there anyone familiar with CYP21A2 gene?

Hello all, I am using panel sequancing and I found a homozygous mutstion in cyp21A2 gene. I did sanger but couldnt find this mutation. I think there is a possibility that I sequanced pseudogene....

09 October 2018 2,952 0 View

Is there anyone who has knowldege in insl3?

Dear all, I would like to know if there is anyone who know a research group or a paper on mouse modeling studying insl3?

09 October 2018 8,855 0 View

What are the bioinfromatic tools that can predict deletions and insertions?

Hello everyone, I am working on some SNPs and mutations and I would like to have good prediction tools. It is easy to find bioinformatic tools for SNPs prediction but I couldn't find prediction...

06 July 2018 3,371 0 View

Can anyone recommend a good booksand programs for biostatistics?

Hello everyone, can you please recommend good books for biostatistics for both beginner level and a little advanced level? I also would like to have or advice for a good satistical progams that...

03 April 2018 375 7 View

Can any one help me with protien stability prediction tool?

Hello every one, I am looking for a bioinformatic tool for protien stability prediction. Can you please suggest for me? or provide me with links? Thank you

02 March 2018 5,688 5 View

Why project HOPE couldnt find the amino acid change?

Hello everyone, we extracted SNPs from dbSNP then we retrived the protien sequance from Uniprot. Then we submitted the amino acids changes corresponding to the protien change to proiect HOPE...

02 March 2018 1,566 1 View

Why Alleles in the same SNP changes?

I would like to know the wild type and mutant type allele of rs1344706 but some time I find it A/C and some times I find it G/T. Is this has anything to do with NCBI assay ID? Will a SNP has the...

02 March 2018 1,794 5 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Victor C. Kok

There is a recently published article using EBV DNA viral load for the blood samples.

Gudrun Lang

We stain mRNA of EBV (EBER) in paraffin section with a commercial kit (CISH). It works well, but is only a yes/no analysis. I have no infos about comparison to sensitivity of blood tests.

Marc-Antoine Perrenoud

Real time PCR works well on both type of samples, with FRET, you just have to prepare the paraffine samples before extraction of DNA.

Gabriela Muñoz

For our lab, real time PCR is fine in both samples and you can use quantitative PCR to better stablish the relationship with disease.