We have both paraffin- waxed tissue samples and blood from patients We would like to know what is the best method to use for the analysis of EBV?
There is a recently published article using EBV DNA viral load for the blood samples.
We stain mRNA of EBV (EBER) in paraffin section with a commercial kit (CISH). It works well, but is only a yes/no analysis. I have no infos about comparison to sensitivity of blood tests.
Real time PCR works well on both type of samples, with FRET, you just have to prepare the paraffine samples before extraction of DNA.
For our lab, real time PCR is fine in both samples and you can use quantitative PCR to better stablish the relationship with disease.
Dear all, i am looking for a list or suggestions for biomdical jurnals that can publish for free or waiver publication cost for authors from low income countries. I know about the BMCs but I need...
04 May 2019 8,833 4 View
Hello everyone, I would like to use RFLP to detect specific SNP. I would like to selelect specific enzyme that cuts in certain nucleaotide using NEBcutter but I do not know if I did well using...
10 November 2018 5,629 0 View
Dear all, Now a days there are too many bioinformatic tools and sftwares freely available on the net like SIFT and Polyphen. These tools are able to predict the effect of specific varaiant on...
09 October 2018 591 2 View
Hello all, I am using panel sequancing and I found a homozygous mutstion in cyp21A2 gene. I did sanger but couldnt find this mutation. I think there is a possibility that I sequanced pseudogene....
09 October 2018 2,831 0 View
Dear all, I would like to know if there is anyone who know a research group or a paper on mouse modeling studying insl3?
09 October 2018 8,725 0 View
Hello everyone, I am working on some SNPs and mutations and I would like to have good prediction tools. It is easy to find bioinformatic tools for SNPs prediction but I couldn't find prediction...
06 July 2018 3,252 0 View
Hello everyone, can you please recommend good books for biostatistics for both beginner level and a little advanced level? I also would like to have or advice for a good satistical progams that...
03 April 2018 222 7 View
Hello everyone, we extracted SNPs from dbSNP then we retrived the protien sequance from Uniprot. Then we submitted the amino acids changes corresponding to the protien change to proiect HOPE...
02 March 2018 1,419 1 View
Hello every one, I am looking for a bioinformatic tool for protien stability prediction. Can you please suggest for me? or provide me with links? Thank you
02 March 2018 5,562 5 View
I would like to know the wild type and mutant type allele of rs1344706 but some time I find it A/C and some times I find it G/T. Is this has anything to do with NCBI assay ID? Will a SNP has the...
02 March 2018 1,675 5 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...
02 March 2021 752 2 View