Do you know journals for free publication cost?

Dear all, i am looking for a list or suggestions for biomdical jurnals that can publish for free or waiver publication cost for authors from low income countries. I know about the BMCs but I need...

04 May 2019 8,833 4 View

How to use NEBcutter?

Hello everyone, I would like to use RFLP to detect specific SNP. I would like to selelect specific enzyme that cuts in certain nucleaotide using NEBcutter but I do not know if I did well using...

10 November 2018 5,629 0 View

What are the parameter of the bioinformatic tools?

Dear all, Now a days there are too many bioinformatic tools and sftwares freely available on the net like SIFT and Polyphen. These tools are able to predict the effect of specific varaiant on...

09 October 2018 591 2 View

Is there anyone who has knowldege in insl3?

Dear all, I would like to know if there is anyone who know a research group or a paper on mouse modeling studying insl3?

09 October 2018 8,725 0 View

What are the bioinfromatic tools that can predict deletions and insertions?

Hello everyone, I am working on some SNPs and mutations and I would like to have good prediction tools. It is easy to find bioinformatic tools for SNPs prediction but I couldn't find prediction...

06 July 2018 3,252 0 View

Can anyone recommend a good booksand programs for biostatistics?

Hello everyone, can you please recommend good books for biostatistics for both beginner level and a little advanced level? I also would like to have or advice for a good satistical progams that...

03 April 2018 222 7 View

Why project HOPE couldnt find the amino acid change?

Hello everyone, we extracted SNPs from dbSNP then we retrived the protien sequance from Uniprot. Then we submitted the amino acids changes corresponding to the protien change to proiect HOPE...

02 March 2018 1,419 1 View

Can any one help me with protien stability prediction tool?

Hello every one, I am looking for a bioinformatic tool for protien stability prediction. Can you please suggest for me? or provide me with links? Thank you

02 March 2018 5,562 5 View

Why Alleles in the same SNP changes?

I would like to know the wild type and mutant type allele of rs1344706 but some time I find it A/C and some times I find it G/T. Is this has anything to do with NCBI assay ID? Will a SNP has the...

02 March 2018 1,675 5 View

What is the molecular weight of hpv e6 protien and scrible?

05 June 2017 2,804 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View