Marc-Antoine Perrenoud

6 Questions 21 Answers 0 Followers

Questions related from Marc-Antoine Perrenoud

Is it possible to get good sequencing results despite having no signal on electrophoresis gel ?

I was performing a 16Srrna Broad Range PCR. Despite having no signal on my electrophoresis gel, I still tried to elonguate and sequence ( Conventional Sanger sequencing ) to get results. I was...

11 November 2018 7,983 6 View

Any advice on the standard for Colony PCR on direct samples ?

Hi Folks, We performed colony PCR on 16S eubacterial gene for a long time, since a bit of time we got small issues with our positive controls, the band aren't so bright and beautiful as before. We...

12 December 2015 6,687 9 View

Does any one has knowledge why phagotherapy is so uncommon in western Europa countries?

Since I read about this on some articles, I can't stop asking myself why such techniques are not widely used in Western Europa Countries? It seems that it is very efficient in Eastern countries...

09 September 2015 8,001 3 View

How can I increase the performance of my Eubacterias PCR on Patients sample?

I explain, i'm doing PCR on Chlamydia trachomatis. The PCR on strains works fine, i got large bands on gel, and when i'm sequencing it, the result is perfect.Then i had to do the PCR on patients...

02 February 2015 9,204 5 View

Does anyone have knowledge of the efficacity of TaKaRa Ex Taq Hot-Start Version kit in large spectre PCR for diagnosis?

I'm trying to optimize a polymerase chain reaction for detection of eubacterias, mycobacterias, among other things. I'm trying several different kits and I wanted to know the pros/contras of this...

11 November 2014 1,994 3 View

What type of Mix (Roche, Applied biosystems, ...) could I use to optimize the time of my home made PCR ( Eubacteria, Mycobacteria) ?

I'm doing work on a new home made PCR for diagnostic use (large spectre detections for eubacteria, mycobacteria, identifications & resistances genes) I need to optimize the time and cycle to...

10 October 2014 6,245 2 View