What is the best method for cDNA synthesis from miRNA?

More Payman sameea yousefi's questions See All

Is it possible to estimate Horizontal in-situ stresses' directions and magnitudes from seismic data by using AI methods?

We know that one of the important members of a 3D Geomechanical Model is in-situ stresses' directions and magnitudes. we can use the seismic data to estimate these parameters in special record...

21 May 2024 1,332 2 View

Why polyacetal homopolymer is produced from formaldehyde? why not from trioxane which is solid at room temperature?

Polyacetal homopolymer is produced from formaldehyde in industrial scale. The monomer is in gasous state and it needs a tedious purification process. Trioxane is an alternative monomer for...

15 April 2024 8,622 0 View

Im runing FPLC (Fast Protein Liquid Chromatograph) my ferritin in ph7 and ph 2 , does anyone knows how can I make sure how much is the retention time?

I have used PEG as supposed it would be stable in ph 2 and ph 7 and by shifting their peak can understand ho much is the retention time, but have nt yet get any good results

24 October 2023 8,236 2 View

Is it possible to form Jiant cells in J774 cell culture medium? What is the cause of this phenomenon?

11 July 2023 4,510 1 View

Does Escherichia coli Nissle 1917 multiply as fast as other E. coli bacteria?

25 April 2023 582 4 View

I need a tip for buying a plasmid extraction kit ?

I'm going to buy a plasmid extraction kit but I don't know which brand has manufactured a good one. Someone recommend the FavorPrep Plasmid Extraction Mini Kit , please if you have used it so far...

31 January 2023 663 0 View

What‘s the particulars of less than 2.5 Micron emited from vehicles that cause air pollution (Hc , No, or other gasses?

the particular matters which are less than 2.5 Micron emitted from vehicles cause air pollution , now which of them is major element I(Hc , No, or other gasses?

29 November 2022 4,754 9 View

How I can use sodium deoxycholate as surfactant?

I need to use sodium deoxycholate (NaDC) as surfactant in a methacrylate polymerization system for preparing hydrogel nanoparticles. But the reaction mixture (in water) precipitates before the...

22 October 2022 9,984 1 View

Safety of probiotics?

Is there a specific safety guidance for isolated probiotics? if not what are the most important in vitro assays to insure the safety of probiotics?

21 October 2022 3,087 3 View

Do you think boiling waters changes nitrate content of water to nitrite and increase nitrite content?

some experts express: boiling waters changes nitrate content of water to nitrite and increase nitrite content so people worried about this issue ,what's your opinion?

25 June 2022 3,027 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View